Metabolomics Investigation of an Association of Induced Features and Corresponding Fungus during the Co-culture of Trametes versicolor and Ganoderma applanatum.
Metabolomics Investigation of an Association of Induced Features and Corresponding Fungus during the Co-culture of Trametes versicolor and Ganoderma applanatum.
复制标题
栓菌与悬垂灵芝共培养过程中诱导特征与相应真菌关联的代谢组学研究
DOI：
10.3389/fmicb.2017.02647
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
Yang S
中科院分区：
文献类型：
--
作者：
Xu XY;Shen XT;Yuan XJ;Zhou YM;Fan H;Zhu LP;Du FY;Sadilek M;Yang J;Qiao B;Yang S
杂色栓菌和树舌菌的共培养是一种强烈的担子菌相互作用的模型，它诱导了许多新合成的或高度产生的特征。目前，主要的挑战之一是识别在共培养过程中诱导特征的起源。在此，我们报告了一种13 C动态标记分析，用于确定诱导特征与相应真菌的关联，即使代谢物的身份不可用或生化方面几乎一无所知。将T.杂色G. applanatum培养10天后，T.杂色G.无菌收获树舌，然后在液体培养基中进行单培养，所述液体培养基含有一半新鲜培养基，13 C标记的葡萄糖作为碳源，并且在第10天收集一半共培养上清液。通过LC-MS分析的13 C标记代谢物组显示，在共培养刺激后的单培养物中，包括3-苯基乳酸和地衣酸的31个诱导特征被同位素标记。从T. versicolor，G. applanatum的5个特征，并通过T.杂色G.树舌13 C标记进一步表明，12个特征，如以前确定的新木糖苷[N-（4-甲氧基苯基）甲酰胺2-O-β-D-木糖苷]可能是通过菌丝体的直接物理相互作用诱导的。使用分子网络分析结合13 C-标记提供了一个深入了解结构类似物的产生和生产真菌之间的联系。m/z为309.0757的化合物1在共培养物中增加了15.4倍，并且在两种T.杂色G. applanatum纯化并鉴定为苯基聚酮化合物，2，5，6-三羟基-4，6-二苯基环己-4-烯-1，3-二酮。生物活性研究表明，该化合物具有抑制白血病细胞系U937细胞活力的潜力。目前的工作为进一步研究奠定了重要基础，包括新的代谢产物的发现和生物合成能力的提高。
The co-culture of Trametes versicolor and Ganoderma applanatum is a model of intense basidiomycete interaction, which induces many newly synthesized or highly produced features. Currently, one of the major challenges is an identification of the origin of induced features during the co-culture. Herein, we report a 13C-dynamic labeling analysis used to determine an association of induced features and corresponding fungus even if the identities of metabolites were not available or almost nothing was known of biochemical aspects. After the co-culture of T. versicolor and G. applanatum for 10 days, the mycelium pellets of T. versicolor and G. applanatum were sterilely harvested and then mono-cultured in the liquid medium containing half fresh medium with 13C-labeled glucose as carbon source and half co-cultured supernatants collected on day 10. 13C-labeled metabolome analyzed by LC-MS revealed that 31 induced features including 3-phenyllactic acid and orsellinic acid were isotopically labeled in the mono-culture after the co-culture stimulation. Twenty features were derived from T. versicolor, 6 from G. applanatum, and 5 features were synthesized by both T. versicolor and G. applanatum. 13C-labeling further suggested that 12 features such as previously identified novel xyloside [N-(4-methoxyphenyl)formamide 2-O-beta-D-xyloside] were likely induced through the direct physical interaction of mycelia. Use of molecular network analysis combined with 13C-labeling provided an insight into the link between the generation of structural analogs and producing fungus. Compound 1 with m/z 309.0757, increased 15.4-fold in the co-culture and observed 13C incorporation in the mono-culture of both T. versicolor and G. applanatum, was purified and identified as a phenyl polyketide, 2,5,6-trihydroxy-4, 6-diphenylcyclohex-4-ene-1,3-dione. The biological activity study indicated that this compound has a potential to inhibit cell viability of leukemic cell line U937. The current work sets an important basis for further investigations including novel metabolites discovery and biosynthetic capacity improvement.
登录
查看更多内容
影响因子：
4.2
作者：
Hiscox J;Savoury M;Toledo S;Kingscott-Edmunds J;Bettridge A;Waili NA;Boddy L
通讯作者：
Boddy L
唤醒沉默的生物合成基因簇以及将孤儿簇与微生物中的天然产物联系起来的最新进展。
影响因子：
7.8
作者：
Chiang, Yi-Ming;Chang, Shu-Lin;Oakley, Berl R.;Wang, Clay C. C.
通讯作者：
Wang, Clay C. C.
DOI：
10.1038/ismej.2015.38
发表时间：
2015-10
期刊：
The ISME journal
影响因子：
--
作者：
Hiscox J;Savoury M;Müller CT;Lindahl BD;Rogers HJ;Boddy L
通讯作者：
Boddy L
影响因子：
5.1
作者：
Ola, Antonius R. B.;Thomy, Dhana;Prolcsch, Peter
通讯作者：
Prolcsch, Peter
影响因子：
--
作者：
Holman, Jerry D;Tabb, David L;Mallick, Parag
通讯作者：
Mallick, Parag