CD4(+)CD25(+) Cells Are Essential for Maintaining Immune Tolerance in Chickens Inoculated with Bovine Serum Albumin at the Late Stage of Embryonic Development.
CD4(+)CD25(+) Cells Are Essential for Maintaining Immune Tolerance in Chickens Inoculated with Bovine Serum Albumin at the Late Stage of Embryonic Development.
复制标题
CD4 CD25 细胞对于在胚胎发育后期接种牛血清白蛋白的鸡维持免疫耐受至关重要
DOI：
10.3390/vetsci7040150
复制
发表时间：
2020-10-03
影响因子：
2.4
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Yu X;Meng Y;Pan P;Li L;Rui L;Li Z
本研究探讨了鸡CD 4 + CD 25+细胞在诱导免疫耐受过程中的作用。对经流式细胞仪分选的鸡CD 4 + CD 25+细胞的特性进行了分析。结果表明，鸡CD 4 + CD 25+细胞在体外高表达IL-10、TGF-β，并抑制CD 4 + CD 25 −细胞的增殖。为了诱导免疫耐受，在胚胎孵育第20天（EID 20），通过血管内途径向胚胎接种牛血清白蛋白（BSA），孵化后的小鸡经历BSA免疫，间隔7天，共4次。分析血清抗BSA抗体和CD 4 + CD 25+细胞比率。结果表明，随着免疫耐受的进展，小鼠获得了体液免疫耐受，外周血淋巴细胞中CD 4 + CD 25+细胞百分率沿着升高。在EID 16时通过血管内途径注射抗鸡CD 25抗体来阻断CD 4 + CD 25+细胞，并且CD 4 + CD 25+细胞比率在孵化后35天显著降低。在此基础上，在EID 16和EID 20分别注射抗鸡CD 25抗体和BSA，并与BSA注射组进行耐受性对比。CD 4 + CD 25+细胞阻断组小鼠血清中抗BSA抗体水平明显升高，表明免疫耐受水平降低。总之，鸡CD 4 + CD 25+细胞在维持诱导的免疫耐受中是必不可少的。
In this study, the role of chicken CD4+CD25+ cells during induced immunotolerance was tested. Properties of chicken CD4+CD25+ cells sorted by flow cytometry were analyzed. Results showed that chicken CD4+CD25+ cells express IL-10, TGF-β highly and suppress proliferation of CD4+CD25− cells in vitro. To induce immunotolerance, embryos were inoculated with bovine serum albumin (BSA) via an intravascular route on embryo incubation day 20 (EID20), and after hatching chicks experienced BSA immunization four times at 7-day intervals. Serum anti-BSA antibodies and CD4+CD25+ cell ratio was analyzed. Results showed that humoral tolerance was obtained and the CD4+CD25+ cell percentage in peripheral blood lymphocytes increased along with this progress. Injection of anti-chicken CD25 antibody via an intravascular route on EID16 is applied to block CD4+CD25+ cells, and the CD4+CD25+ cell ratio decreased significantly up to 35 d post-hatch. Based on the above, injections of anti-chicken CD25 antibody on EID16 and BSA on EID20 were carried out sequentially, and tolerance level was contrasted to the BSA-injection group. Data revealed the anti-BSA antibodies increased significantly in the CD4+CD25+ cell-blocked groups indicating that immune tolerance level was weakened. In conclusion, chicken CD4+CD25+ cells are essential in maintaining induced immune tolerance.
登录
查看更多内容
DOI：
10.1073/pnas.1015148108
发表时间：
2011-02-08
影响因子：
11.1
作者：
Dietze, Kirsten K.;Zelinskyy, Gennadiy;Dittmer, Ulf
通讯作者：
Dittmer, Ulf
影响因子：
2.9
作者：
Selvaraj, Ramesh K.
通讯作者：
Selvaraj, Ramesh K.
影响因子：
30.5
作者：
Apostolou, I;Sarukhan, A;von Boehmer, H
通讯作者：
von Boehmer, H
影响因子：
5.8
作者：
Wen Z;Wang X;Dong K;Zhang H;Bu Z;Ye L;Yang C
通讯作者：
Yang C
影响因子：
8
作者：
Workman, Creg J.;Szymczak-Workman, Andrea L.;Collison, Lauren W.;Pillai, Meenu R.;Vignali, Dario A. A.
通讯作者：
Vignali, Dario A. A.