Vitellogenin induction in caudal fin of guppy (Poecilia reticulata) as a less invasive and sensitive biomarker for environmental estrogens.
Vitellogenin induction in caudal fin of guppy (Poecilia reticulata) as a less invasive and sensitive biomarker for environmental estrogens.
复制标题
孔雀鱼 (Poecilia reticulata) 尾鳍中的卵黄蛋白原诱导作为环境雌激素的侵入性较小且敏感的生物标志物
DOI：
10.1038/s41598-017-06670-6
复制
发表时间：
2017-08-09
影响因子：
4.6
通讯作者：
Ru S
中科院分区：
文献类型：
--
作者：
Wang J;Ma S;Zhang Z;Zheng M;Dong Y;Ru S
孔雀鱼（Poecilia reticulata）是研究环境雌激素的理想模型，其巨大的尾鳍具有很高的再生能力。本研究分析了尾鳍用于检测卵黄蛋白原（Vtg）（最常用的环境雌激素生物标志物）的可行性。首先，使用纯化的卵黄蛋白及其抗体开发了孔雀鱼Vtg夹心ELISA，其工作范围为7.8-1000ng/mL，检测限为3.1ng/mL。采用ELISA法检测Vtg的组织分布。暴露于50和100ng/L 17β-雌二醇（E2）的雄性孔雀鱼，尾鳍中的Vtg浓度高于全鱼、大脑、眼睛、性腺和皮肤，并且与肝脏中的Vtg浓度接近。此外，将雄性孔雀鱼暴露于环境浓度的 17a-炔雌醇 (EE2) 和双酚 S (BPS) 中，以验证尾鳍 Vtg 在检测雌激素活性方面的效用。 EE2和BPS的最低观察到的效应浓度分别低于2ng/L和1μg/L，低于或等于其他物种报道的值，表明尾鳍Vtg对雌激素化学物质高度敏感。因此，建议将孔雀鱼的尾鳍作为 Vtg 生物标志物检测的替代样本。
Guppy (Poecilia reticulata) is an ideal model for studying environmental estrogens, and its large caudal fin has a high capacity to regenerate. This study analyzed the feasibility of caudal fin for detecting vitellogenin (Vtg), the most commonly used biomarker of environmental estrogens. Firstly, a sandwich ELISA for guppy Vtg was developed using purified lipovitellin and its antibody and it had a working range of 7.8–1000 ng/mL and detection limit of 3.1 ng/mL. The ELISA was used to detect tissue distribution of Vtg. In male guppy exposed to 50 and 100 ng/L 17β-estradiol (E2), Vtg concentration in caudal fin was higher than that in whole fish, brain, eyes, gonad, and skin, and was close to that in the liver. Furthermore, male guppies were exposed to environmental concentrations of 17a-ethinylestradiol (EE2) and bisphenol S (BPS) to validate the utility of caudal fin Vtg for detecting estrogenic activities. The lowest observed effect concentration of EE2and BPS were lower than 2 ng/L and 1 μg/L, which were below or equal to the values reported for other species, demonstrating that caudal fin Vtg was highly sensitive to estrogenic chemicals. Therefore, caudal fins of guppies are suggested as alternative samples for Vtg biomarker detection.
登录
查看更多内容
DOI：
10.1016/s1532-0456(01)00194-6
发表时间：
2001-07-01
影响因子：
3.9
作者：
Fenske, M;van Aerle, R;Segner, H
通讯作者：
Segner, H
影响因子：
4.5
作者：
Folmar, LC;Hemmer, M;Denslow, ND
通讯作者：
Denslow, ND
影响因子：
6.8
作者：
Maltais, Domynick;Roy, Robert L.
通讯作者：
Roy, Robert L.
影响因子：
4.5
作者：
Mortensen, Anne S.;Arukwe, Augustine
通讯作者：
Arukwe, Augustine
影响因子：
4.5
作者：
Bourrachot, Stephanie;Brion, Francois;Adam-Guillermin, Christelle
通讯作者：
Adam-Guillermin, Christelle