Nanoparticles To Study Lectins in Caenorhabditis elegans: Multivalent Galactose β1-4 Fucose-Functionalized Dendrimers Provide Protection from Oxidative Stress.
Nanoparticles To Study Lectins in Caenorhabditis elegans: Multivalent Galactose β1-4 Fucose-Functionalized Dendrimers Provide Protection from Oxidative Stress.
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DOI：
10.1021/acs.biomac.1c01001
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发表时间：
2021-11-08
影响因子：
6.2
通讯作者：
Cloninger MJ
中科院分区：
文献类型：
--
作者：
VanKoten HW;Moore RS;Cloninger MJ
半乳糖凝集素是半乳糖苷结合凝集素，是功能性二聚体或更高级的寡聚体。多价结合已被证明可以增强半乳糖凝集素与其半乳糖苷结合伴侣相对较低的亲和力，使半乳糖凝集素能够在包括心脏病和癌症在内的疾病状态应激条件下发生的细胞整体重塑中发挥重要作用。线虫秀丽隐杆线虫中半乳糖凝集素的存在及其半乳糖苷结合特性已得到证实，但秀丽隐杆线虫半乳糖凝集素的多价相互作用的作用尚不清楚。在这里，我们描述了 Galβ1-4Fuc 功能化聚（酰胺基胺）（PAMAM）树枝状聚合物的合成及其在氧化应激过程中使用秀丽隐杆线虫的研究中的用途。线虫被喂食 Galβ1-4Fuc 功能化树枝状聚合物和 RNA 干扰，以在经历氧化应激时敲低凝集素 lec-1 和 lec-10。用糖树枝状聚合物预处理的线虫比未处理的对照更不易受到氧化应激的影响。喂食荧光标记的糖树枝状聚合物的蠕虫并成像表明，树枝状聚合物主要存在于蠕虫的消化道中，并且在某些情况下也可以观察到外阴和近端性腺的摄取。这项研究表明，多价存在的 Galβ1-4Fuc 可以保护秀丽隐杆线虫免受氧化应激。
Galectins are galactoside-binding lectins that are functional dimers or higher order oligomers. Multivalent binding has been shown to augment the relatively low affinity of the galectins for their galactoside-binding partners, enabling the galectins to play an important role in the global remodeling of cells that occurs during the stress conditions of disease states including heart disease and cancer. The presence of galectins in the nematode Caenorhabditis elegans and their galactoside binding properties have been demonstrated, but the role of multivalent interactions for C. elegans galectins is unknown. Here, we describe the synthesis of Galβ1-4Fuc functionalized poly(amidoamine) (PAMAM) dendrimers and their utility in studies using C. elegans during oxidative stress. C. elegans were fed Galβ1-4Fuc functionalized dendrimers and RNA interference to knock down lectins lec-1 and lec-10 while undergoing oxidative stress. C. elegans that were pre-treated with the glycodendrimers were less susceptible to oxidative stress than untreated controls. Worms fed fluorescently-tagged glycodendrimers and imaged indicated that the dendrimers are primarily present in the digestive tract of the worms, and uptake into the vulva and proximal gonads could also be observed in some instances. This study suggests that multivalently presented Galβ1-4Fuc can protect C. elegans from oxidative stress.
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影响因子：
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