Cholinergic neurons of the basal forebrain mediate biochemical and electrophysiological mechanisms underlying sleep homeostasis.
Cholinergic neurons of the basal forebrain mediate biochemical and electrophysiological mechanisms underlying sleep homeostasis.
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DOI：
10.1111/ejn.12766
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发表时间：
2015-01
期刊：
影响因子：
--
通讯作者：
Basheer R
中科院分区：
文献类型：
--
作者：
Kalinchuk AV;Porkka-Heiskanen T;McCarley RW;Basheer R
睡眠剥夺（sleep deprivation，SD）引起的稳态睡眠压（homeostatic sleep pressure，HSP）是生理生化机制和电生理机制紧密结合的结果。我们曾报道在SD期间，基底前脑（BF）中诱导型一氧化氮合酶（iNOS）、细胞外一氧化氮（NO）、腺苷[AD]ex、乳酸[Lac]ex和丙酮酸[Pyr]ex的水平增加。然而，尚不清楚是否所有这些都有助于HSP导致SD后非快速眼动（NREM）恢复睡眠（RS）期间脑电图（EEG）δ活动增加。以前，我们表明，NREM δ增加明显RS期间取决于BF胆碱能（ChBF）神经元的存在。在这里，我们调查的作用，ChBF细胞在协调的生化和脑电图的变化，在SD和RS大鼠。在SD期间，低θ功率（5- 7 Hz）的增加，而不是高θ（7- 9 Hz），与RS期间NREM δ功率的增加以及硝酸盐/亚硝酸盐[NOx]ex和[AD]ex的变化相关。在SD期间，使用IgG 192-皂草素的ChBF细胞损伤防止了[NOx]ex、[AD]ex和低θ活性的增加，但没有防止[Lac]ex和[Pyr]ex的增加。将NO供体DETA NONOate输注到皂草素处理的BF中未能增加NREM RS和δ功率，表明ChBF细胞对于介导NO稳态作用是重要的。最后，SD诱导的iNOS主要在ChBF细胞中表达，并且iNOS诱导的强度与低theta活性的增加相关。总之，我们的数据表明，ChBF细胞在调节HSP的生化和EEG机制方面很重要。
The tight coordination of biochemical and electrophysiological mechanisms underlies the homeostatic sleep pressure (HSP) produced by sleep deprivation (SD). We have reported that during SD the levels of inducible nitric oxide synthase (iNOS), extracellular nitric oxide (NO), adenosine [AD]ex, lactate [Lac]ex and pyruvate [Pyr]ex increase in the basal forebrain (BF). However, it is not clear whether all of them contribute to HSP leading to increased electroencephalogram (EEG) delta activity during non-rapid eye movement (NREM) recovery sleep (RS) following SD. Previously, we showed that NREM delta increase evident during RS depends on the presence of BF cholinergic (ChBF) neurons. Here, we investigated the role of ChBF cells in coordination of biochemical and EEG changes seen during SD and RS in the rat. Increases in low theta power (5–7Hz), but not high theta (7–9Hz), during SD correlated with the increase in NREM delta power during RS, and with the changes in nitrate/nitrite [NOx]ex and [AD]ex. Lesions of ChBF cells using IgG 192-saporin prevented increases in [NOx]ex, [AD]ex and low theta activity, during SD, but did not prevent increases in [Lac]ex and [Pyr]ex. Infusion of NO donor DETA NONOate into the saporin-treated BF failed to increase NREM RS and delta power, suggesting ChBF cells are important for mediating NO homeostatic effects. Finally, SD-induced iNOS was mostly expressed in ChBF cells, and the intensity of iNOS induction correlated with the increase in low theta activity. Together, our data indicate ChBF cells are important in regulating the biochemical and EEG mechanisms that contribute to HSP.
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DOI：
10.1523/jneurosci.1423-10.2010
发表时间：
2010-06-30
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Dworak M;McCarley RW;Kim T;Kalinchuk AV;Basheer R
通讯作者：
Basheer R
影响因子：
3.4
作者：
Berntson, GG;Shafi, R;Sarter, M
通讯作者：
Sarter, M
DOI：
10.1016/s0169-328x(99)00219-3
发表时间：
1999-11-10
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Basheer, R;Porkka-Heiskanen, T;McCarley, RW
通讯作者：
McCarley, RW
影响因子：
5.5
作者：
Caesar, Kirsten;Hashemi, Parastoo;Lauritzen, Martin
通讯作者：
Lauritzen, Martin
影响因子：
3.3
作者：
Arrigoni, E.;Chamberlin, N. L.;McCarley, R. W.
通讯作者：
McCarley, R. W.