Unravelling the Evolution of the Allatostatin-Type A, KISS and Galanin Peptide-Receptor Gene Families in Bilaterians: Insights from Anopheles Mosquitoes.
Unravelling the Evolution of the Allatostatin-Type A, KISS and Galanin Peptide-Receptor Gene Families in Bilaterians: Insights from Anopheles Mosquitoes.
复制标题
DOI：
10.1371/journal.pone.0130347
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Cardoso JC
中科院分区：
文献类型：
--
作者：
Felix RC;Trindade M;Pires IR;Fonseca VG;Martins RS;Silveira H;Power DM;Cardoso JC
Allatostatin type A receptor（AST-ARs）是一类由FGL-酰胺（AST-A）肽家族成员激活的G-蛋白偶联受体，可抑制节肢动物的摄食和发育。尽管它们的生理重要性的AST-A系统的演变是很少描述和相对较少的受体已被分离和功能特征的昆虫。本研究提供了一个全面的分析的起源和比较演变的AST-A系统。为了确定进化和进食如何改变AST-AR的功能，对按蚊中的复制受体进行了表征。系统发育和基因同线性表明，无脊椎动物AST-A受体和肽基因与KISS/GAL受体和配体有着共同的进化起源。AST-ARs和KISSR是在两侧染色体组GALRs分化后从一个共同的基因祖先中出现的。在节肢动物中，AST-A系统通过谱系特异性事件进化，并且在苍蝇和蚊子（双翅目）中维持两种受体是基因复制事件的结果。按蚊的物种形成影响受体基因的组织和表征的AST-AR重复（GPRALS 1和2）显示，与其他昆虫一样，蚊子受体被激活的昆虫AST-A肽和iCa 2+信号通路刺激。GPRALS 1和GPRALS 2主要在蚊子中肠和卵巢中表达，并且这两种受体的转录丰度通过进食而改变。与葡萄糖喂养的雌性相比，血餐强烈上调中肠中两种GPRALS的表达（p < 0.05）。基于这些结果，我们推测昆虫的AST-A系统与脊椎动物的KISS系统有着共同的起源，并且可能也具有作为代谢和生殖的整合者的共同功能。亮点：AST-A和KISS/GAL受体和配体在原口目-后口目分歧之前具有共同的祖先。系统发育和基因共线性分析表明，AST-AR和KISSR是在GALR基因分化后出现的。AST-AR基因存在于半索动物中，但在脊索动物中丢失。在原口类中，AST-ARs通过谱系特异性事件持续存在和进化，并在节肢动物辐射中复制。双翅目获得并维持功能上不同的重复AST-AR基因。
Allatostatin type A receptors (AST-ARs) are a group of G-protein coupled receptors activated by members of the FGL-amide (AST-A) peptide family that inhibit food intake and development in arthropods. Despite their physiological importance the evolution of the AST-A system is poorly described and relatively few receptors have been isolated and functionally characterised in insects. The present study provides a comprehensive analysis of the origin and comparative evolution of the AST-A system. To determine how evolution and feeding modified the function of AST-AR the duplicate receptors in Anopheles mosquitoes, were characterised. Phylogeny and gene synteny suggested that invertebrate AST-A receptors and peptide genes shared a common evolutionary origin with KISS/GAL receptors and ligands. AST-ARs and KISSR emerged from a common gene ancestor after the divergence of GALRs in the bilaterian genome. In arthropods, the AST-A system evolved through lineage-specific events and the maintenance of two receptors in the flies and mosquitoes (Diptera) was the result of a gene duplication event. Speciation of Anopheles mosquitoes affected receptor gene organisation and characterisation of AST-AR duplicates (GPRALS1 and 2) revealed that in common with other insects, the mosquito receptors were activated by insect AST-A peptides and the iCa2+-signalling pathway was stimulated. GPRALS1 and 2 were expressed mainly in mosquito midgut and ovaries and transcript abundance of both receptors was modified by feeding. A blood meal strongly up-regulated expression of both GPRALS in the midgut (p < 0.05) compared to glucose fed females. Based on the results we hypothesise that the AST-A system in insects shared a common origin with the vertebrate KISS system and may also share a common function as an integrator of metabolism and reproduction. Highlights: AST-A and KISS/GAL receptors and ligands shared common ancestry prior to the protostome-deuterostome divergence. Phylogeny and gene synteny revealed that AST-AR and KISSR emerged after GALR gene divergence. AST-AR genes were present in the hemichordates but were lost from the chordates. In protostomes, AST-ARs persisted and evolved through lineage-specific events and duplicated in the arthropod radiation. Diptera acquired and maintained functionally divergent duplicate AST-AR genes.
登录
查看更多内容
影响因子：
5.2
作者：
Caers J;Verlinden H;Zels S;Vandersmissen HP;Vuerinckx K;Schoofs L
通讯作者：
Schoofs L
影响因子：
--
作者：
BELLES, X;MAESTRO, JL;THORPE, A
通讯作者：
THORPE, A
影响因子：
4.4
作者：
Dana AN;Hong YS;Kern MK;Hillenmeyer ME;Harker BW;Lobo NF;Hogan JR;Romans P;Collins FH
通讯作者：
Collins FH
影响因子：
3.8
作者：
Davey, M;Duve, H;East, P
通讯作者：
East, P
影响因子：
6.5
作者：
Anisimova, Maria;Gascuel, Olivier
通讯作者：
Gascuel, Olivier