Ubiquitous distribution and different subcellular localization of sorbitol dehydrogenase in fruit and leaf of apple.
Ubiquitous distribution and different subcellular localization of sorbitol dehydrogenase in fruit and leaf of apple.
复制标题
苹果果实和叶片中山梨醇脱氢酶的普遍分布和不同亚细胞定位
DOI：
10.1093/jxb/ern347
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发表时间：
2009
影响因子：
6.9
通讯作者：
Gao XQ
中科院分区：
文献类型：
--
作者：
Wang XL;Xu YH;Peng CC;Fan RC;Gao XQ
NAD+依赖性山梨糖醇脱氢酶（nadh - sdh, EC 1.1.1.14）是山梨糖醇代谢的关键酶，在调节苹果果实的贮藏强度和决定果实品质中起重要作用。了解nad1 - sdh的组织和亚细胞定位有助于了解苹果中山梨糖醇的代谢。本研究从苹果果实（Malus domestica Borkh cv.）中分离到两个nadh - sdh cDNA序列。并命名为MdSDH5和MdSDH6。免疫组化分析结果显示，NAD-SDH在果实的果肉和维管组织以及幼叶和老叶的维管组织和叶肉组织中均有分布，表明它是一种在汇源器官中均有表达的蛋白。免疫金电镜分析表明，nadd - sdh主要定位于果实和叶片的细胞质和叶绿体中。通过MdSDH5-GFP和MdSDH6-GFP在拟南芥叶肉原生质体中的瞬时表达，证实了NAD-SDH的叶绿体定位。在幼叶和成熟叶的电子不透明液泡沉积物中也发现了nadh - sdh。这些数据表明，NAD-SDH在果实和叶片中具有不同的亚细胞定位，表明它可能在苹果不同组织中山梨糖醇代谢中发挥不同的作用。
NAD+-dependent sorbitol dehydrogenase (NAD-SDH, EC 1.1.1.14), a key enzyme in sorbitol metabolism, plays an important role in regulating sink strength and determining the quality of apple fruit. Understanding the tissue and subcellular localization of NAD-SDH is helpful for understanding sorbitol metabolism in the apple. In this study, two NAD-SDH cDNA sequences were isolated from apple fruits (Malus domestica Borkh cv. Starkrimson) and named MdSDH5 and MdSDH6. Immunohistochemical analysis revealed that NAD-SDH is distributed in both the flesh and the vascular tissue of the fruit, and the vascular tissue and mesophyll tissue in the young and old leaves, indicating that it is a ubiquitous protein expressed in both sink and source organs. Immunogold electron microscopy analysis demonstrated that NAD-SDH is localized mainly in the cytoplasm and chloroplast of the fruit and leaves. The chloroplast localization of NAD-SDH was confirmed by the transient expression of MdSDH5-GFP and MdSDH6-GFP in the mesophyll protoplast of Arabidopsis. NAD-SDH was also found in electron opaque deposits of vacuoles in young and mature leaves. These data show that NAD-SDH has different subcellular localizations in fruit and leaves, indicating that it might play a different role in sorbitol metabolism in different tissues of apple.
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影响因子：
6.4
作者：
Archbold, DD
通讯作者：
Archbold, DD
影响因子：
5.2
作者：
Park, SW;Song, KJ;Chung, WI
通讯作者：
Chung, WI
重组蛇毒原金属蛋白酶的表达、重折叠和体外激活
影响因子：
1.6
作者：
Ramos, OHP;Carmona, AK;Selistre-de-Araujo, HS
通讯作者：
Selistre-de-Araujo, HS
影响因子：
4.3
作者：
Shen, YY;Duan, CQ;Zhang, DP
通讯作者：
Zhang, DP
DOI：
10.2503/jjshs.68.1099
发表时间：
1999-11-01
影响因子：
--
作者：
Yamada, K;Mori, H;Yamaki, S
通讯作者：
Yamaki, S