Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening
Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening
复制标题
与果实软化相关的草莓果胶甲酯酶的全基因组鉴定和功能表征
DOI：
10.1186/s12870-019-2225-9
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发表时间：
2020-01
影响因子：
5.3
通讯作者：
Xu Chen
中科院分区：
文献类型：
--
作者：
Cheng Xue;Si-Cong Guan;Jian-Qing Chen;Chen-Jin Wen;Jian-Fa Cai;Xu Chen
果胶甲基酯酶（PME）是一种水解酶，可催化高半乳糖醛酸的去甲基酯化，并控制果胶的重建，在细胞壁修饰的调节中起重要作用。果实成熟过程中，pme介导的细胞壁重塑是决定果实硬度和软化的重要过程。草莓果实是一种柔软的水果，采后寿命短，因为坚硬的质地会迅速丧失。因此，采前提高草莓果实硬度是延长果实保鲜时间的前提。虽然PME已经在模式植物中得到了很好的表征，但关于草莓中pmegene家族的功能和进化特性的知识仍然有限。结果林地草莓（Fragaria vesca ' hawaii 4 '）共鉴定出54个pmeme基因（FvPMEs）。系统发育和基因结构分析将这些efvpmegenes分为4个类群（Group 1-4）。重复事件分析表明，串联重复和分散重复有效地促进了草莓PME家族的扩展。通过转录组分析，我们发现fvpme38和fvpme39是果实成熟阶段表达最丰富的pmes，它们受到脱落酸的正调控。通过过表达和rnai沉默基因操纵offvpme38和fvpme39显著影响果实硬度、果胶含量和细胞壁结构，表明草莓果实软化需要PME。结论本研究通过系统发育、进化预测和遗传分析等方法对草莓果胶甲基酯酶进行了全面分析。我们验证了offvpme38和fvpme39在草莓果实软化过程中的重要调控作用，为通过调节PME水平来提高草莓果实硬度提供了指导。
BackgroundPectin methylesterase (PME) is a hydrolytic enzyme that catalyzes the demethylesterification of homogalacturonans and controls pectin reconstruction, being essential in regulation of cell wall modification. During fruit ripening stage, PME-mediated cell wall remodeling is an important process to determine fruit firmness and softening. Strawberry fruit is a soft fruit with a short postharvest life, due to a rapid loss of firm texture. Hence, preharvest improvement of strawberry fruit rigidity is a prerequisite for extension of fruit refreshing time. Although PME has been well characterized in model plants, knowledge regarding the functionality and evolutionary property ofPMEgene family in strawberry remain limited.ResultsA total of 54PMEgenes (FvPMEs) were identified in woodland strawberry (Fragaria vesca‘Hawaii 4’). Phylogeny and gene structure analysis divided theseFvPMEgenes into four groups (Group 1–4). Duplicate events analysis suggested that tandem and dispersed duplications effectively contributed to the expansion of the PME family in strawberry. Through transcriptome analysis, we identifiedFvPME38andFvPME39as the most abundant-expressedPMEs at fruit ripening stages, and they were positively regulated by abscisic acid. Genetic manipulation ofFvPME38andFvPME39by overexpression and RNAi-silencing significantly influences the fruit firmness, pectin content and cell wall structure, indicating a requirement of PME for strawberry fruit softening.ConclusionOur study globally analyzed strawberry pectin methylesterases by the approaches of phylogenetics, evolutionary prediction and genetic analysis. We verified the essential role ofFvPME38andFvPME39in regulation of strawberry fruit softening process, which provided a guide for improving strawberry fruit firmness by modifying PME level.
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影响因子：
6.7
作者：
Dorokhov YL;Komarova TV;Petrunia IV;Frolova OY;Pozdyshev DV;Gleba YY
通讯作者：
Gleba YY
影响因子：
16.6
作者：
通讯作者：
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草莓果实中的瞬时表达测定
影响因子：
0.8
作者：
Mengting Pi;Qi Gao;Chunying Kang
通讯作者：
Chunying Kang
影响因子：
4.3
作者：
Louvet, Romain;Cavel, Emilie;Pelloux, Jerome
通讯作者：
Pelloux, Jerome
影响因子：
4.1
作者：
Hasunuma, T;Fukusaki, E;Kobayashi, A
通讯作者：
Kobayashi, A