Differences in time-dependent mechanical properties between extruded and molded hydrogels.
Differences in time-dependent mechanical properties between extruded and molded hydrogels.
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DOI：
10.1088/1758-5090/8/3/035012
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发表时间：
2016-08-22
期刊：
影响因子：
9
通讯作者：
Spiller KL
中科院分区：
文献类型：
--
作者：
Ersumo N;Witherel CE;Spiller KL
用于生物材料和组织工程应用的水凝胶的机械性能是其功能的关键决定因素。尽管添加剂制造，特别是基于挤压的生物印刷作为一种重要的生物制造方法最近兴起，但对挤压结构的力学行为的全面研究仍然缺乏。为了解决这一知识差距，我们比较了通过传统成型技术和使用Biobots Beta气动挤出机进行3D生物打印制备的交联明胶水凝胶的机械性能和溶胀性能。初步表征了生物打印参数对结构性能的影响，结果表明，杨氏模数和最佳挤出压力都随着聚合物含量的增加而增加，打印分辨率随着打印速度和喷嘴厚度的增加而提高。包裹的NIH3T3成纤维细胞的高存活率(>；95%)证实了构建体制备工艺的细胞相容性。有趣的是，挤压和模塑结构的杨氏模数没有什么不同，但挤压结构确实在蠕变过程中观察到的随时间变化的力学行为的速度和程度都有所增加。尽管聚合物密度相似，但与模制水凝胶相比，挤出水凝胶随着时间的推移表现出更大的膨胀率，这表明蠕变行为的差异源于微观结构和流体流动的不同。由于与时间相关的机械性能、流体流动和膨胀性能对组织和细胞行为的关键作用，这些发现强调了更多地考虑挤压过程对水凝胶性能的影响的必要性。
The mechanical properties of hydrogels used in biomaterials and tissue engineering applications are critical determinants of their functionality. Despite the recent rise of additive manufacturing, and specifically extrusion-based bioprinting, as a prominent biofabrication method, comprehensive studies investigating the mechanical behavior of extruded constructs remain lacking. To address this gap in knowledge, we compared the mechanical properties and swelling properties of crosslinked gelatin-based hydrogels prepared by conventional molding techniques or by 3D bioprinting using a BioBots Beta pneumatic extruder. A preliminary characterization of the impact of bioprinting parameters on construct properties revealed that both Young's modulus and optimal extruding pressure increased with polymer content, and that printing resolution increased with both printing speed and nozzle gauge. High viability (>95%) of encapsulated NIH 3T3 fibroblasts confirmed the cytocompatibility of the construct preparation process. Interestingly, the Young's moduli of extruded and molded constructs were not different, but extruded constructs did show increases in both the rate and extent of time-dependent mechanical behavior observed in creep. Despite similar polymer densities, extruded hydrogels showed greater swelling over time compared to molded hydrogels, suggesting that differences in creep behavior derived from differences in microstructure and fluid flow. Because of the crucial roles of time-dependent mechanical properties, fluid flow, and swelling properties on tissue and cell behavior, these findings highlight the need for greater consideration of the effects of the extrusion process on hydrogel properties.
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影响因子：
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作者：
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