Twist-1 regulates the miR-199a/214 cluster during development.
Twist-1 regulates the miR-199a/214 cluster during development.
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Twist-1在开发过程中调节miR-199a/214群集。
DOI：
10.1093/nar/gkn920
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发表时间：
2009-01
影响因子：
14.9
通讯作者：
Uney JB
中科院分区：
文献类型：
--
作者：
Lee YB;Bantounas I;Lee DY;Phylactou L;Caldwell MA;Uney JB
已知microRNA调节发育过程，但其调节机制在很大程度上仍不清楚。我们显示转录因子Twist-1驱动编码miR-199 a和miR-214簇的7.9 kb非编码RNA转录物（来自发动蛋白-3基因内含子）的表达。我们还发现，用shRNAs敲低Twist-1降低了miR-199 a/214水平，Twist-1结合了E-Box启动子基序，从而在发育过程中调节这些miRNAs的表达。HIF-1（已知介导Twist-1转录）、miR-199 a和miR-214的表达在E12.5达到最大，并且miRNAs特异性地表达于小鼠小脑、中脑、鼻突和前后肢芽。这项研究表明，miR 199 a/214簇的表达是由Twist-1通过E-Box启动子元件控制的，并支持这些miRNA作为控制特定神经细胞群发育的途径中的新型中间体的作用。
MicroRNAs are known to regulate developmental processes but their mechanism of regulation remains largely uncharacterized. We show the transcription factor Twist-1 drives the expression of a 7.9-kb noncoding RNA transcript (from the Dynamin-3 gene intron) that encodes a miR-199a and miR-214 cluster. We also show that knocking down Twist-1 with shRNAs decreased miR-199a/214 levels and that Twist-1 bound an E-Box promoter motif to developmentally regulate the expression of these miRNAs. The expression of HIF-1 (known to mediate Twist-1 transcription), miR-199a and miR-214 was maximal at E12.5 and the miRNAs were expressed specifically in mouse cerebellum, midbrain, nasal process and fore- and hindlimb buds. This study shows the expression of the miR199a/214 cluster is controlled by Twist-1 via an E-Box promoter element and supports a role for these miRNAs as novel intermediates in the pathways controlling the development of specific neural cell populations.
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影响因子：
56.9
作者：
Spicer, DB;Rhee, J;Lassar, AB
通讯作者：
Lassar, AB
影响因子：
4.8
作者：
Kim, Seonhoe;Lee, Ui Jin;Choi, Young-Chul
通讯作者：
Choi, Young-Chul
影响因子：
2.7
作者：
Gitelman, I
通讯作者：
Gitelman, I
DOI：
10.1073/pnas.0705917104
发表时间：
2007-09-18
影响因子：
11.1
作者：
Chakrabarty, Anindita;Tranguch, Susanne;Dey, Sudhansu K.
通讯作者：
Dey, Sudhansu K.
影响因子：
4.4
作者：
Loebel, DAF;Tsoi, B;Tam, PPL
通讯作者：
Tam, PPL