Five serum microRNAs for detection and predicting of ovarian cancer
Five serum microRNAs for detection and predicting of ovarian cancer
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用于检测和预测卵巢癌的五种血清 microRNA
DOI：
10.1016/j.eurox.2019.100017
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发表时间：
2019-04
影响因子：
--
通讯作者：
Wei Zhu
中科院分区：
文献类型：
--
作者：
Weiwei Wang;Li-rong Wu;Chunyu Li;Xin Zhou;Ping Liu;Xuemei Jia;Yan Chen;Wei Zhu
卵巢癌（Ovarian cancer，OC）是全球女性最致命的妇科恶性肿瘤之一。血清microRNAs（miRNAs）可作为OC患者有前途的诊断生物标志物。研究设计采用基于qRT-PCR的RT-qon panel，我们在初始筛选阶段从一个正常对照（NC）池和两个OC池样本中鉴定了27个差异表达的miRNAs。我们基于qRT-PCR进一步验证了训练阶段（30 OC vs. 36 NC）和验证阶段（80 OC vs. 80 NC）的miRNA。结果我们发现，与NC相比，OC中存在5种过表达的miRNAs（miR-200 c-3 p，miR-346，miR-127- 3 p，miR-143- 3 p和miR-205- 5 p）。五种miRNA组的受试者工作特征（ROC）曲线下面积（AUC）在训练阶段为0.783，在验证阶段为0.745。当临界值为0.636时，组合的五种miRNA组的诊断灵敏度和特异性分别为0.818和0.609。血清中miR-200 c-3 p、miR-346和miR-127- 3 p水平与OC的分级和远处转移有关。5种miRNA的表达水平在血清外泌体中也显著上调（32 OC vs. 32 NC）。此外，miR-200 c-3 p在OC组织中显著升高（22 OC vs. 22 NC）。而miR-346和miR-143- 3 p在OC组织中的表达水平显著降低。血清中miR-200 c-3 p、miR-346和miR-127- 3 p的表达水平与卵巢癌的分级和远处转移有关。
ObjectiveOvarian cancer (OC) was one of the deadliest gynecological malignancy among women in global. Serum microRNAs (miRNAs) could serve as promising diagnostic biomarkers for patients with OC.Study designUsing quantitative reverse transcription polymerase chain reaction (qRT-PCR) based Exiqon panel, we identified 27 differentially expressed miRNAs from one normal control (NC) pool and two OC pool samples in the initial screening stage. We further verified the miRNAs in the training (30 OC VS. 36 NCs) and validation stages (80 OC VS. 80 NCs) based on qRT-PCR. Later, the expression levels of the identified miRNAs were also evaluated in exosomes and tissues.ResultsWe found a serum microRNA signature including five overexpressed miRNAs (miR-200c-3p, miR-346, miR-127-3p, miR-143-3p and miR-205-5p) in OC in comparison with NCs. The areas under the receiver operating characteristic (ROC) curve (AUC) of the five-miRNA panel were 0.783 for the training stage and 0.745 for the validation stage. The diagnostic sensitivity and specificity of the combined five-miRNA panel was 0.818 and 0.609 when the cut-off value was 0.636. The levels of miR-200c-3p, miR-346 and miR-127-3p in serum were related to tumor grade and distant metastasis of OC. The expression levels of the five miRNAs were also significantly up-regulated in serum exosomes (32 OC VS. 32 NCs). Furthermore, miR-200c-3p was significantly elevated in OC tissues (22 OC VS. 22 NCs). But the levels of the miR-346 and miR-143-3p were significantly lower in OC tissues.ConclusionOur findings showed a five-miRNA panel in serum for the detection of OC. Moreover, serum expression levels of miR-200c-3p, miR-346 and miR-127-3p were concerned with tumor grade and distant metastasis of OC.
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DOI：
10.1891/9780826121646.0002
发表时间：
2018-09
期刊：
Cancer Rehabilitation
影响因子：
--
作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
通讯作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
影响因子：
--
作者：
Kinose Y;Sawada K;Nakamura K;Kimura T
通讯作者：
Kimura T
影响因子：
2.6
作者：
Wan WN;Zhang YQ;Wang XM;Liu YJ;Zhang YX;Que YH;Zhao WJ;Li P
通讯作者：
Li P
影响因子：
4.2
作者：
Hongyan Zhang;Wanbin Li
通讯作者：
Hongyan Zhang;Wanbin Li
影响因子：
2
作者：
Corney DC;Nikitin AY
通讯作者：
Nikitin AY