Comparative evaluation of nanofibrous scaffolding for bone regeneration in critical-size calvarial defects.
Comparative evaluation of nanofibrous scaffolding for bone regeneration in critical-size calvarial defects.
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DOI：
10.1089/ten.tea.2008.0433
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发表时间：
2009-08
影响因子：
--
通讯作者：
Ma PX
中科院分区：
文献类型：
--
作者：
Woo KM;Chen VJ;Jung HM;Kim TI;Shin HI;Baek JH;Ryoo HM;Ma PX
In a previous study we found that nano-fibrous poly(L-lactic acid) scaffolds mimicking collagen fibers in size were superior to solid-walled scaffolds in promoting osteoblast differentiation and bone formation in vitro. In this study we used an in vivo model to confirm the biological properties of nano-fibrous poly(L-lactic acid) scaffolds and to evaluate how effectively they support bone regeneration against solid-walled scaffolds. The scaffolds were implanted in critical size defects made on rat calvarial bones. Compared with solid-walled scaffolds, nano-fibrous scaffolds supported substantially more new bone tissue formation, which was confirmed by micro-CT measurement and von Kossa staining. Goldner’s trichrome staining showed abundant collagen deposition in nano-fibrous scaffolds but not in the control solid-walled scaffolds. The cells in these scaffolds were immuno-stained strongly for Runx2 and bone sialoprotein (BSP). In contrast, solid-walled scaffolds implanted in the defects were stained weakly with trichrome, Runx2 and BSP. These in vivo results demonstrate that nano-fibrous architecture enhances osteoblast differentiation and bone formation.
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影响因子：
--
作者：
Ma, PX;Choi, JW
通讯作者：
Choi, JW
影响因子：
14
作者：
Wei, Guobao;Jin, Qiming;Ma, Peter X.
通讯作者：
Ma, Peter X.
影响因子：
14
作者：
Woo, Kyung Mi;Jun, Ji-Hae;Ma, Peter X.
通讯作者：
Ma, Peter X.
影响因子：
14
作者：
Yoshimoto, H;Shin, YM;Vacanti, JP
通讯作者：
Vacanti, JP
影响因子：
3.6
作者：
Gosain, AK;Santoro, TD;Madoub, HS
通讯作者：
Madoub, HS