High iron-mediated increased oral fungal burden, oral-to-gut transmission, and changes to pathogenicity of Candida albicans in oropharyngeal candidiasis.
High iron-mediated increased oral fungal burden, oral-to-gut transmission, and changes to pathogenicity of Candida albicans in oropharyngeal candidiasis.
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DOI：
10.1080/20002297.2022.2044110
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发表时间：
2022
影响因子：
4.5
通讯作者：
Puri S
中科院分区：
文献类型：
--
作者：
Tripathi A;Nahar A;Sharma R;Kanaskie T;Al-Hebshi N;Puri S
铁影响口腔微生物景观的多样性。引起口咽念珠菌病（OPC）的白色念珠菌的致病性菌株CAI 4在体外表现出铁诱导的细胞壁变化，影响吞噬作用（通过巨噬细胞）和真菌细胞对细胞壁扰动抗真菌剂的易感性。了解铁对CAI 4株、野生型（WT）SC 5314株和口腔分离株的影响。白色念珠菌。使用OPC的免疫抑制小鼠模型来评估铁对CAI 4-菌株的口-肠感染和抗真菌药敏性的影响。在体外抗真菌药敏试验，细胞壁分析，并进行吞噬试验低铁和高铁，为SC 5314株和口腔分离株。高铁增强了小鼠中CAI 4菌株的口腔和肠道真菌水平;来自低铁小鼠的CAI 4细胞对抗真菌药物更敏感。SC 5314菌株和口腔分离株对大多数抗真菌药的耐药性增强，高铁。铁介导的细胞壁的变化和吞噬反应的SC 5315-菌株是类似的CAI 4;口腔分离株表现出可变的反应。宿主铁可以潜在地改变感染的严重程度和传播，抗真菌治疗的疗效，以及OPC期间的宿主免疫应答。临床分离株表现出铁的这些影响，尽管表现出不同的细胞壁组成变化的反应，铁。
Iron affects the diversity of the oral microbial landscape. Laboratory-strain CAI4 of Candida albicans that causes oropharyngeal candidiasis (OPC) exhibits iron-induced changes to the cell wall, impacting phagocytosis (by macrophages) and susceptibility of fungal cells to cell wall-perturbing antifungals, in vitro. To understand the effect of iron on the CAI4-strain, wild type (WT) SC5314-strain, and oral isolates of C. albicans. An immunosuppressed murine model of OPC was used to assess the effect of iron on oral-to-gut infection and antifungal susceptibility of the CAI4-strain. In vitro antifungal susceptibility, cell wall analysis, and phagocytic assays were performed under low and high iron, for the SC5314-strain and oral isolates. High iron enhanced oral and gut fungal levels for the CAI4-strain in mice; CAI4 cells from low iron mice were more susceptible to antifungals. The SC5314-strain and oral isolates showed enhanced antifungal-resistance towards most antifungals tested, under high iron. Iron-mediated cell wall changes and phagocytic response in the SC5315-strain were similar to CAI4; oral isolates showed a variable response. Host iron can potentially alter infection severity and dissemination, efficacy of antifungal treatment, and host immune response during OPC. Clinical isolates showed most of these effects of iron, despite exhibiting a varied cell wall composition-change response to iron.
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DOI：
10.3390/jof3040051
发表时间：
2017-09-23
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
--
作者：
Hernández-Chávez MJ;Pérez-García LA;Niño-Vega GA;Mora-Montes HM
通讯作者：
Mora-Montes HM
影响因子：
11.8
作者：
Nucci, M;Anaissie, E
通讯作者：
Anaissie, E
影响因子：
16.6
作者：
Pradhan, Arnab;Avelar, Gabriela M.;Brown, Alistair J. P.
通讯作者：
Brown, Alistair J. P.
影响因子：
6.7
作者：
Hopke A;Nicke N;Hidu EE;Degani G;Popolo L;Wheeler RT
通讯作者：
Wheeler RT
影响因子：
3.6
作者：
Puri S;Lai WK;Rizzo JM;Buck MJ;Edgerton M
通讯作者：
Edgerton M