Cell-mediated and humoral immune responses in pigs following primary and challenge-exposure to Lawsonia intracellularis.
Cell-mediated and humoral immune responses in pigs following primary and challenge-exposure to Lawsonia intracellularis.
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DOI：
10.1186/1297-9716-43-9
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发表时间：
2012-02-08
影响因子：
4.4
通讯作者：
Jungersen G
中科院分区：
文献类型：
--
作者：
Cordes H;Riber U;Jensen TK;Jungersen G
为了研究再次感染胞内劳森氏菌后的免疫应答，在22头猪中研究了对初次和攻毒接种的局部和外周体液和细胞介导的免疫应答。猪经口接种L.在5-6周龄时接种细胞内感染，用抗生素治疗，并在12周龄时用再接种（RE）攻击。在12周龄时，处理对照（TC）猪仅接受初次接种，攻毒对照（CC）猪仅接受二次接种。在该方案后，所有RE猪均受到保护，免于再感染，如通过肠粘膜的定殖和病理学减少、不存在细菌脱落和血清急性期蛋白应答不增加所定义的。在受保护的RE猪中，血清IgG应答在高应答者和低应答者中是可变的。血清伊加反应没有加强的再接种，因为相同的肠道伊加反应中开发的易感CC猪和受保护的RE猪接种。RE猪中出现了记忆回忆细胞介导的免疫反应，通过全血IFN-γ测定和流式细胞术后计算IFN-γ积分中位荧光强度（iMFI）评估，与年龄匹配的CC猪的主要反应相比，该反应显着更强。IFN-γ产生细胞主要为CD 8+和CD 4 + CD 8+双阳性淋巴细胞。结果表明，细胞介导的免疫应答可能是抗乳酸杆菌保护性免疫的介质。在细胞内，CD 8+效应细胞和CD 4 + CD 8+双阳性记忆T细胞是抗原特异性IFN-γ产生的主要贡献者。
To investigate immune responses upon re-infection with Lawsonia intracellularis, local and peripheral humoral and cell-mediated immune responses to primary and challenge inoculations were studied in 22 pigs. Pigs were orally inoculated with virulent L. intracellularis at the age of 5-6 weeks, treated with antibiotics and challenged with a re-inoculation (RE) at the age of 12 weeks. Treatment control (TC) pigs received only the primary inoculation and challenge control (CC) pigs received only the secondary inoculation at 12 weeks of age. Following this regimen, all RE pigs were protected against the re-infection as defined by reduced colonisation and pathology of intestinal mucosa, absence of bacterial shedding and without increase in serum acute phase protein response. In the protected RE pigs, serum IgG responses were variable with both high and low responders. Serum IgA responses were not boosted by the re-inoculation, since identical intestinal IgA responses developed in response to the inoculation in both the susceptible CC pigs and the protected RE pigs. A memory recall cell-mediated immune response developed in RE pigs which was significantly stronger compared to the primary response in age-matched CC pigs as assessed by whole blood IFN-γ assay and by calculation of IFN-γ integrated median fluorescence intensity (iMFI) after flow cytometry. The major IFN-γ producing cells were identified as CD8+ and CD4+CD8+ double positive lymphocytes. The results indicate that cell-mediated immune responses are likely mediators of protective immunity against L. intracellularis, with CD8+ effector cells and CD4+CD8+ double positive memory T cells as main contributors to the antigen-specific IFN-γ production.
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影响因子：
3.3
作者：
Riber, Ulla;Cordes, Henriette;Jungersen, Gregers
通讯作者：
Jungersen, Gregers
影响因子：
1
作者：
Kroll, JJ;Roof, MB;McOrist, S
通讯作者：
McOrist, S
影响因子：
3.1
作者：
HANEBERG, B;KENDALL, D;NEUTRA, MR
通讯作者：
NEUTRA, MR
影响因子：
3.3
作者：
Jacobson, M.;Aspan, A.;Wallgren, P.
通讯作者：
Wallgren, P.
影响因子：
3.1
作者：
MCORIST, S;MACINTYRE, N;LAWSON, GHK
通讯作者：
LAWSON, GHK