PIRIN2 stabilizes cysteine protease XCP2 and increases susceptibility to the vascular pathogen Ralstonia solanacearum in Arabidopsis.
PIRIN2 stabilizes cysteine protease XCP2 and increases susceptibility to the vascular pathogen Ralstonia solanacearum in Arabidopsis.
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DOI：
10.1111/tpj.12602
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Tuominen H
中科院分区：
文献类型：
--
作者：
Zhang B;Tremousaygue D;Denancé N;van Esse HP;Hörger AC;Dabos P;Goffner D;Thomma BP;van der Hoorn RA;Tuominen H
PIRIN （PRN）是功能多样的杯蛋白超家族的一员。拟南芥PRN家族有四个成员，但这些蛋白的作用在很大程度上是未知的。本文描述了拟南芥PIRIN2 （PRN2）的一个功能，该功能与植物细菌病原菌茄枯菌的易感性有关。两个prn2突变等位基因对茄青霉感染的反应显示出疾病发展和细菌生长的下降。通过分析PRN2与木瓜样半胱氨酸蛋白酶（PLCPs） XCP2、RD21A和RD21B的相互作用，我们阐明了潜在的分子机制，这些酶在酵母双杂交实验和拟南芥原生质体共免疫沉淀实验中都与PRN2结合。基于PLCP活性分析和重组蛋白酶促分析，我们发现PRN2通过抑制XCP2的自溶来稳定XCP2。在植物中也证实了PRN2对XCP2的稳定作用。与prn2突变体一样，xcp2单敲除突变体和xcp2 prn2双敲除突变体对茄枯病菌的易感性降低，这表明prn2稳定xcp2是拟南芥对茄枯病菌易感性的基础。
PIRIN (PRN) is a member of the functionally diverse cupin protein superfamily. There are four members of the Arabidopsis thaliana PRN family, but the roles of these proteins are largely unknown. Here we describe a function of the Arabidopsis PIRIN2 (PRN2) that is related to susceptibility to the bacterial plant pathogen Ralstonia solanacearum. Two prn2 mutant alleles displayed decreased disease development and bacterial growth in response to R. solanacearum infection. We elucidated the underlying molecular mechanism by analyzing PRN2 interactions with the papain-like cysteine proteases (PLCPs) XCP2, RD21A, and RD21B, all of which bound to PRN2 in yeast two-hybrid assays and in Arabidopsis protoplast co-immunoprecipitation assays. We show that XCP2 is stabilized by PRN2 through inhibition of its autolysis on the basis of PLCP activity profiling assays and enzymatic assays with recombinant protein. The stabilization of XCP2 by PRN2 was also confirmed in planta. Like prn2 mutants, an xcp2 single knockout mutant and xcp2 prn2 double knockout mutant displayed decreased susceptibility to R. solanacearum, suggesting that stabilization of XCP2 by PRN2 underlies susceptibility to R. solanacearum in Arabidopsis.
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