A survey to evaluate parameters governing the selection and application of extracellular vesicle isolation methods.
A survey to evaluate parameters governing the selection and application of extracellular vesicle isolation methods.
复制标题
评价控制细胞外囊泡分离方法的选择和应用的参数的调查。
DOI：
10.1177/20417314231155114
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发表时间：
2023-01
影响因子：
8.2
通讯作者：
Davies, Owen G.
中科院分区：
文献类型：
--
作者：
Williams, Soraya;Jalal, Aveen R.;Lewis, Mark P.;Davies, Owen G.
细胞外囊泡（EV）在诊断和治疗应用方面继续引起科学界的兴趣。随着EV应用的多样化，研究人员必须意识到挑战，特别是EV分离方法与下游应用及其临床转化的兼容性。我们报告了第一次交叉比较研究的结果，该研究旨在确定控制跨学科流行EV隔离方法选择的参数（EV源，起始体积，运营商经验，应用和实施参数，如成本和可扩展性）。我们的研究结果强调了临床关注的增加，36%的受访者将电动汽车应用于治疗和诊断。数据表明，优先选择超离心用于治疗应用，沉淀试剂在临床设置和尺寸排阻色谱用于诊断应用，利用生物流体。方法选择受操作员经验的影响，当EV研究不是受访者的主要关注点时，方法多样性增加。应用和实施标准被认为是方法选择的主要影响因素，UC和SEC分别因其处理大体积和小体积的能力而被选择。总的来说，我们确定了影响EV科学范围内方法选择的参数，为有效转化研究成果提供了有价值的实用考虑因素概述。
Extracellular vesicles (EVs) continue to gain interest across the scientific community for diagnostic and therapeutic applications. As EV applications diversify, it is essential that researchers are aware of challenges, in particular the compatibility of EV isolation methods with downstream applications and their clinical translation. We report outcomes of the first cross-comparison study looking to determine parameters (EV source, starting volume, operator experience, application and implementation parameters such as cost and scalability) governing the selection of popular EV isolation methods across disciplines. Our findings highlighted an increased clinical focus, with 36% of respondents applying EVs in therapeutics and diagnostics. Data indicated preferential selection of ultracentrifugation for therapeutic applications, precipitation reagents in clinical settings and size exclusion chromatography for diagnostic applications utilising biofluids. Method selection was influenced by operator experience, with increased method diversity when EV research was not the respondents primary focus. Application and implementation criteria were indicated to be major influencers in method selection, with UC and SEC chosen for their abilities to process large and small volumes, respectively. Overall, we identified parameters influencing method selection across the breadth of EV science, providing a valuable overview of practical considerations for the effective translation of research outcomes.
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影响因子：
3.9
作者：
通讯作者：
--
影响因子：
16
作者：
Barreiro K;Dwivedi OP;Leparc G;Rolser M;Delic D;Forsblom C;Groop PH;Groop L;Huber TB;Puhka M;Holthofer H
通讯作者：
Holthofer H
影响因子：
16
作者：
Clayton, Aled;Boilard, Eric;Nieuwland, Rienk
通讯作者：
Nieuwland, Rienk
影响因子：
5.5
作者：
Gu C;Feng J;Waqas A;Deng Y;Zhang Y;Chen W;Long J;Huang S;Chen L
通讯作者：
Chen L
尿细胞外囊泡：国际细胞外囊泡学会尿液工作组的立场文件。
影响因子：
16
作者：
Erdbrügger U;Blijdorp CJ;Bijnsdorp IV;Borràs FE;Burger D;Bussolati B;Byrd JB;Clayton A;Dear JW;Falcón-Pérez JM;Grange C;Hill AF;Holthöfer H;Hoorn EJ;Jenster G;Jimenez CR;Junker K;Klein J;Knepper MA;Koritzinsky EH;Luther JM;Lenassi M;Leivo J;Mertens I;Musante L;Oeyen E;Puhka M;van Royen ME;Sánchez C;Soekmadji C;Thongboonkerd V;van Steijn V;Verhaegh G;Webber JP;Witwer K;Yuen PST;Zheng L;Llorente A;Martens-Uzunova ES
通讯作者：
Martens-Uzunova ES