Acute respiratory distress syndrome induced by H9N2 virus in mice.
Acute respiratory distress syndrome induced by H9N2 virus in mice.
复制标题
H9N2病毒诱发小鼠急性呼吸窘迫综合征
DOI：
10.1007/s00705-009-0560-0
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发表时间：
2010-03
影响因子：
2.7
通讯作者：
Qiao J
中科院分区：
文献类型：
--
作者：
Deng G;Bi J;Kong F;Li X;Xu Q;Dong J;Zhang M;Zhao L;Luan Z;Lv N;Qiao J
H9 N2禽流感病毒在一些国家多次引起猪和人类感染。本研究的目的是评估H9 N2病毒感染小鼠引起的肺病理学变化。用1 × 104 MID 50的A/Chicken/Hebei/4/2008（H9 N2）病毒鼻内感染6 - 8周龄BALB/c小鼠。观察感染后不同时间点的临床症状、肺组织病理变化、病毒复制情况、动脉血气及支气管肺泡灌洗液（BALF）中细胞因子的变化。对照组用非感染性尿囊液鼻内感染。H9 N2感染的小鼠表现出严重的呼吸综合征，死亡率为60%。大体观察显示，受感染的肺高度水肿。感染肺的主要组织病理学变化包括弥漫性肺炎和肺泡损伤，伴嗜中性粒细胞为主的炎性细胞浸润、间质和肺泡水肿、出血和重度细支气管炎/细支气管周围炎。此外，H9 N2病毒感染导致严重进行性低氧血症、淋巴细胞减少以及BALF中中性粒细胞、肿瘤坏死因子-α和白细胞介素-6的显著增加。上述特征符合急性呼吸窘迫综合征（ARDS）的标准。我们的数据表明，H9 N2病毒感染导致小鼠ARDS，这可能有助于研究未来潜在的H9 N2疾病在人类的发病机制。
H9N2 avian influenza viruses have repeatedly caused infections in swine and humans in some countries. The purpose of the present study was to evaluate the pulmonary pathology caused by H9N2 viral infection in mice. Six- to eight-week-old BALB/c mice were infected intranasally with 1 × 104 MID50 of A/Chicken/Hebei/4/2008(H9N2) virus. Clinical signs, pathological changes and viral replication in lungs, arterial blood gas, and cytokines in bronchoalveolar lavage fluid (BALF) were observed at different time points after infection. A control group was infected intranasally with noninfectious allantoic fluid. H9N2-infected mice exhibited severe respiratory syndrome, with a mortality rate of 60%. Gross observations showed that infected lungs were highly edematous. Major histopathological changes in infected lungs included diffuse pneumonia and alveolar damage, with neutrophil-dominant inflammatory cellular infiltration, interstitial and alveolar edema, hemorrhage, and severe bronchiolitis/peribronchiolitis. In addition, H9N2 viral infection resulted in severe progressive hypoxemia, lymphopenia, and a significant increase in neutrophils, tumor necrosis factor-α and interleukin-6 in BALF. The features described above satisfy the criteria for acute respiratory distress syndrome (ARDS). Our data show that H9N2 viral infection resulted in ARDS in mice, and this may facilitate studies of the pathogenesis of future potential H9N2 disease in humans.
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影响因子：
6
作者：
Majeski, EI;Paintlia, MK;London, L
通讯作者：
London, L
影响因子：
24.3
作者：
Pelosi, P;D'Onofrio, D;Gattinoni, L
通讯作者：
Gattinoni, L
影响因子：
9.4
作者：
Butt, KM;Smith, GJD;Guan, Y
通讯作者：
Guan, Y
影响因子：
3.3
作者：
Ng WF;To KF;Lam WW;Ng TK;Lee KC
通讯作者：
Lee KC
DOI：
10.1073/pnas.160270697
发表时间：
2000-08-15
影响因子：
11.1
作者：
Lin, YP;Shaw, M;Hay, A
通讯作者：
Hay, A