High-throughput microarray reveals the epitranscriptome-wide landscape of m(6)A-modified circRNA in oral squamous cell carcinoma.
High-throughput microarray reveals the epitranscriptome-wide landscape of m(6)A-modified circRNA in oral squamous cell carcinoma.
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高通量微阵列揭示口腔鳞状细胞癌中 m6A 修饰的 circRNA 的全表观转录组景观
DOI：
10.1186/s12864-022-08806-z
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发表时间：
2022-08-23
期刊：
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
新出现的转录组范围的高通量筛选揭示了RNAs的景观和功能，如环状RNAs(CircRNAs)，在人类癌症中。此外，RNA转录后的内部修饰，特别是N6-甲基腺苷(M6A)，极大地丰富了RNA代谢的多样性。然而，CircRNAs上的m6A修饰尚未解决。在这里，我们报告了口腔鳞状细胞癌(OSCC)中m6A修饰的CircRNA(m6A-CircRNA)的表位转录范围的定位。利用m6A甲基化RNA免疫沉淀测序(MERIP-seq)和m6A-CircRNAs微阵列的数据，我们发现m6A-CircRNAs在口腔鳞癌中表现出独特的修饰方式，这种修饰方式不依赖于m6A-mRNA。此外，编码序列(CDS)前部的长外显子经常发生m6A修饰，这与3‘-非编码区或终止密码子中的m6A-mRNA不同。综上所述，我们的工作初步揭示了m6A-CircRNAs的特性，这可能会对m6A-CircRNAs在口腔鳞癌中的作用带来启示。网上版载有补充材料，可在10.1186/s12864-022-08806-z查阅。1.M6A-CircRNAs在口腔鳞状细胞癌中表现出其独特的修饰方式，这种修饰方式不依赖于m6A-mRNA。2.CircRNA上的m6A多出现在CDS前部的长外显子上，这与3‘-UTR或终止密码子中的m6A-mRNA不同。网上版载有补充材料，可在10.1186/s12864-022-08806-z查阅。
Emerging transcriptome-wide high-throughput screenings reveal the landscape and functions of RNAs, such as circular RNAs (circRNAs), in human cancer. In addition, the post-transcriptional RNA internal modifications, especially N6-methyladenosine (m6A), greatly enrich the variety of RNAs metabolism. However, the m6A modification on circRNAs has yet to be addressed. Here, we report an epitranscriptome-wide mapping of m6A-modified circRNAs (m6A-circRNA) in oral squamous cell carcinoma (OSCC). Utilizing the data of m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) and m6A-circRNAs microarray, we found that m6A-circRNAs exhibited particular modification styles in OSCC, which was independent of m6A-mRNA. Besides, m6A modification on circRNAs frequently occurred on the long exons in the front part of the coding sequence (CDS), which was distinct from m6A-mRNA that in 3’-UTR or stop codon. In conclusion, our work preliminarily demonstrates the traits of m6A-circRNAs, which may bring enlighten for the roles of m6A-circRNAs in OSCC. The online version contains supplementary material available at 10.1186/s12864-022-08806-z. 1. m6A-circRNAs exhibited their particular modification style in OSCC, which was independent of m6A-mRNA. 2. m6A on circRNAs frequently occurred on the long exons in the front part of CDS, which was distinct from m6A-mRNA that in 3’-UTR or stop codon. The online version contains supplementary material available at 10.1186/s12864-022-08806-z.
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