Characterization of retinal ganglion cell, horizontal cell, and amacrine cell types expressing the neurotrophic receptor tyrosine kinase Ret.
Characterization of retinal ganglion cell, horizontal cell, and amacrine cell types expressing the neurotrophic receptor tyrosine kinase Ret.
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DOI：
10.1002/cne.24367
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发表时间：
2018-03-01
期刊：
影响因子：
--
通讯作者：
Badea TC
中科院分区：
文献类型：
--
作者：
Parmhans N;Sajgo S;Niu J;Luo W;Badea TC
我们报道了在发育过程中和成年小鼠中，Ret（一种胶质细胞源性神经营养因子（GDNF）家族配体（GFLs）的受体酪氨酸激酶）的视网膜表达模式。Ret最初在视网膜神经节细胞（RGCs）中表达，随后在水平细胞（HCs）和无长突细胞（ACs）中表达，从有丝分裂后发育的早期阶段开始。在成年个体中，Ret在这三类神经元中持续表达。通过RNA测序、免疫染色和随机稀疏重组，我们表明Ret在至少三种不同类型的无长突细胞以及十种类型的视网膜神经节细胞中表达。利用交叉遗传学，我们描述了与POU4f/Brn3转录因子家族的三个成员中的每一个结合表达Ret的视网膜神经节细胞类型的树突分支形态。Ret的表达分别与4种视网膜神经节细胞类型中的Brn3a、5种中的Brn3b以及1种中的Brn3c重叠。Ret⁺视网膜神经节细胞投射到外侧膝状体核（LGN）、顶盖前区（PTA）和上丘（SC），并且避开视交叉上核和副视系统。Brn3a⁺Ret⁺和Brn3c⁺Ret⁺视网膜神经节细胞优先投射到对侧视网膜接受区，而Brn3b⁺Ret⁺视网膜神经节细胞显示出少量同侧投射到橄榄顶盖前核和外侧膝状体核。我们的研究结果建立了用于单个Ret⁺视网膜神经节细胞类型的解剖学和发育特征描述的交叉遗传学方法。此外，它们为解决GDNF神经营养信号传导和转录调控在视网膜神经节细胞类型特化中的潜在相互作用提供了必要信息。
We report the retinal expression pattern of Ret, a receptor tyrosine kinase for the glial derived neurotrophic factor (GDNF) family ligands (GFLs), during development and in the adult mouse. Ret is initially expressed in retinal ganglion cells (RGCs), followed by horizontal cells (HCs) and amacrine cells (ACs), beginning with the early stages of postmitotic development. Ret expression persists in all three classes of neurons in the adult. Using RNA sequencing, immunostaining and random sparse recombination, we show that Ret is expressed in at least three distinct types of ACs, and ten types of RGCs. Using intersectional genetics, we describe the dendritic arbor morphologies of RGC types expressing Ret in combination with each of the three members of the POU4f/Brn3 family of transcription factors. Ret expression overlaps with Brn3a in 4 RGC types, with Brn3b in 5 RGC types, and with Brn3c in one RGC type, respectively. Ret+ RGCs project to the lateral geniculate nucleus (LGN), pretectal area (PTA) and superior colliculus (SC), and avoid the suprachiasmatic nucleus and accessory optic system. Brn3a+ Ret+ and Brn3c+ Ret+ RGCs project preferentially to contralateral retinorecipient areas, while Brn3b+ Ret+ RGCs shows minor ipsilateral projections to the olivary pretectal nucleus and the LGN. Our findings establish intersectional genetic approaches for the anatomic and developmental characterization of individual Ret+ RGC types. In addition, they provide necessary information for addressing the potential interplay between GDNF neurotrophic signaling and transcriptional regulation in RGC type specification.
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DOI：
10.1523/jneurosci.1218-09.2009
发表时间：
2009-07-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Crook JD;Davenport CM;Peterson BB;Packer OS;Detwiler PB;Dacey DM
通讯作者：
Dacey DM
影响因子：
3.7
作者：
Badea TC;Hua ZL;Smallwood PM;Williams J;Rotolo T;Ye X;Nathans J
通讯作者：
Nathans J
DOI：
10.1523/jneurosci.2555-10.2011
发表时间：
2011-05-18
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Cherry TJ;Wang S;Bormuth I;Schwab M;Olson J;Cepko CL
通讯作者：
Cepko CL
影响因子：
5.5
作者：
CLELAND, BG;LEVICK, WR
通讯作者：
LEVICK, WR
影响因子：
64.8
作者：
DACEY, DM;LEE, BB
通讯作者：
LEE, BB