Genes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.
Genes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.
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DOI：
10.1371/journal.pone.0097100
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Browning GF
中科院分区：
文献类型：
--
作者：
Sharma S;Markham PF;Browning GF
支原体被认为是研究生物体独立生存所需的最低遗传补充的有用模型。牛支原体（Mycoplasma bovis）是一种全球性分布的病原体，可引起肺炎、乳腺炎、关节炎、中耳炎和生殖道疾病，目前已发表了3株牛支原体（Mycoplasma bovis）的基因组序列，其中模式株PG 45和2株中国分离株。在这项研究中，产生了几个基于Tn4001的转座子构建体，并用于创建M.牛PG45插入突变体文库。直接基因组测序的319个独立的插入检测到129个基因在M。其中48株与丝状支原体丝状亚种SC有同源性，99株与无乳支原体有同源性。发现在先前对其他支原体种属的研究中发现的16个基因是必需的。这些基因中的五个先前被预测为支原体中153个必需基因的核心组的一部分。因此，这项研究扩大了支原体的非必需基因的名单，从以前的研究中产生的其他物种。
Mycoplasmas are regarded to be useful models for studying the minimum genetic complement required for independent survival of an organism. Mycoplasma bovis is a globally distributed pathogen causing pneumonia, mastitis, arthritis, otitis media and reproductive tract disease, and genome sequences of three strains, the type strain PG45 and two strains isolated in China, have been published. In this study, several Tn4001 based transposon constructs were generated and used to create a M. bovis PG45 insertional mutant library. Direct genome sequencing of 319 independent insertions detected disruptions in 129 genes in M. bovis, 48 of which had homologues in Mycoplasma mycoides subspecies mycoides SC and 99 of which had homologues in Mycoplasma agalactiae. Sixteen genes found to be essential in previous studies on other mycoplasma species were found to be dispensable. Five of these genes have previously been predicted to be part of the core set of 153 essential genes in mycoplasmas. Thus this study has extended the list of non-essential genes of mycoplasmas from that previously generated by studies in other species.
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