Excessive Accumulation of Intracellular Ca(2+) After Acute Exercise Potentiated Impairment of T-cell Function.
Excessive Accumulation of Intracellular Ca(2+) After Acute Exercise Potentiated Impairment of T-cell Function.
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DOI：
10.3389/fphys.2021.728625
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发表时间：
2021
影响因子：
4
通讯作者：
Mooren FC
中科院分区：
文献类型：
--
作者：
Liu R;Krüger K;Pilat C;Fan W;Xiao Y;Seimetz M;Ringseis R;Baumgart-Vogt E;Eder K;Weissmann N;Mooren FC
Ca2+ 是一种重要的细胞内第二信使，已知可调节多种细胞功能。本研究旨在通过测量细胞内钙水平、Ca2+调节基因表达和激动剂诱发的小鼠脾T淋巴细胞增殖来研究运动引起的免疫抑制的机制。小鼠被随机分配到对照组、久坐组（C）和三个实验组，这三个实验组进行了单次强化和力竭的跑步机运动。运动后立即（E0）、3小时（E3）和24小时（E24）通过密度梯度离心分离小鼠脾淋巴细胞。 Fura-2/AM 用于监测活细胞中细胞质游离 Ca2+ 浓度。采用羧基荧光素二乙酸琥珀酰亚胺酯(CFSE)标记和流式细胞术联合方法检测T细胞增殖。使用 qPCR 定量 Ca2+ 调节基因的转录水平。 E3 组的基础细胞内 Ca2+ 水平和激动剂（ConA、OKT3 或毒胡萝卜素）诱导的 Ca2+ 瞬变均显着升高（与对照组相比，p<0.05）。然而，E3 组有丝分裂原诱导的细胞增殖显着降低（与对照组相比，p<0.05）。与此同时，E3 中质膜 Ca2+-ATP 酶 (PMCA)、肌/内质网 Ca2+-ATP 酶 (SERCA)、TRPC1 和 P2X7 的转录水平显着下调，IP3R2 和 RyR2 的转录水平显着上调（与对照相比，p<0.01）。总之，这项研究表明，剧烈运动影响细胞内钙稳态，最有可能是通过增强跨膜 Ca2+ 流入细胞和减少 Ca2+-ATP 酶（如 PMCA 和 SERCA）的表达。然而，改变的 Ca2+ 信号并未转导为增强的 T 细胞增殖，这表明其他途径导致了短暂的运动相关免疫抑制。
Ca2+ is an important intracellular second messenger known to regulate several cellular functions. This research aimed to investigate the mechanisms of exercise-induced immunosuppression by measuring intracellular calcium levels, Ca2+-regulating gene expression, and agonist-evoked proliferation of murine splenic T lymphocytes. Mice were randomly assigned to the control, sedentary group (C), and three experimental groups, which performed a single bout of intensive and exhaustive treadmill exercise. Murine splenic lymphocytes were separated by density-gradient centrifugation immediately (E0), 3h (E3), and 24h after exercise (E24). Fura-2/AM was used to monitor cytoplasmic free Ca2+ concentration in living cells. The combined method of carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling and flow cytometry was used for the detection of T cell proliferation. The transcriptional level of Ca2+-regulating genes was quantified by using qPCR. Both basal intracellular Ca2+ levels and agonist (ConA, OKT3, or thapsigargin)-induced Ca2+ transients were significantly elevated at E3 group (p<0.05 vs. control). However, mitogen-induced cell proliferation was significantly decreased at E3 group (p<0.05 vs. control). In parallel, the transcriptional level of plasma membrane Ca2+-ATPases (PMCA), sarco/endoplasmic reticulum Ca2+-ATPases (SERCA), TRPC1, and P2X7 was significantly downregulated, and the transcriptional level of IP3R2 and RyR2 was significantly upregulated in E3 (p<0.01 vs. control). In summary, this study demonstrated that acute exercise affected intracellular calcium homeostasis, most likely by enhancing transmembrane Ca2+ influx into cells and by reducing expression of Ca2+-ATPases such as PMCA and SERCA. However, altered Ca2+ signals were not transduced into an enhanced T cell proliferation suggesting other pathways to be responsible for the transient exercise-associated immunosuppression.
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DOI：
10.1016/0005-2736(89)90210-1
发表时间：
1989-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
OHTA, A;MOHRI, T;OHYASHIKI, T
通讯作者：
OHYASHIKI, T
影响因子：
5.3
作者：
Mazzeo, RS;Rajkumar, C;Esler, M
通讯作者：
Esler, M
钙和ROS：相互互动。
影响因子：
11.4
作者：
Görlach A;Bertram K;Hudecova S;Krizanova O
通讯作者：
Krizanova O
影响因子：
5.5
作者：
Bautista, DM;Hoth, M;Lewis, RS
通讯作者：
Lewis, RS
跑步机跑步和芦丁对饮食诱导肥胖小鼠脂肪组织中脂肪分解信号通路和 TRPV4 蛋白表达的影响。
影响因子：
3.4
作者：
Chen, Neng;Cheng, Jinbo;Wan, Zhongxiao
通讯作者：
Wan, Zhongxiao