The Arabidopsis wall associated kinase-like 10 gene encodes a functional guanylyl cyclase and is co-expressed with pathogen defense related genes.
The Arabidopsis wall associated kinase-like 10 gene encodes a functional guanylyl cyclase and is co-expressed with pathogen defense related genes.
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DOI：
10.1371/journal.pone.0008904
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发表时间：
2010-01-26
期刊：
影响因子：
3.7
通讯作者：
Gehring C
中科院分区：
文献类型：
--
作者：
Meier S;Ruzvidzo O;Morse M;Donaldson L;Kwezi L;Gehring C
第二，信使在将环境刺激与生理反应联系起来方面起着关键作用。鸟苷3‘，5’-环单磷酸(CGMP)是其中的一种信使，长期以来被认为是高等植物许多不同生理过程中的重要信号分子，包括生物胁迫反应。然而，到目前为止，在高等植物中催化GTP形成cGMP的鸟苷酸环化酶(GC)在很大程度上仍然难以捉摸。我们已经确定了一个拟南芥受体型壁相关激酶样分子(AtWAKL10)作为候选GC，并提供了实验证据表明AtWAKL10431-700的胞内结构域在体外可以产生cGMP。此外，我们还证明了该分子具有激酶活性，表明AtWAKL10是一种双域催化蛋白。共表达和刺激特异性表达分析表明，AtWAKL10与病原菌防御相关基因一致共表达，并与这些基因一起在一系列病原体及其激发子的诱导下被早期和急剧地诱导。我们证明了AtWAKL10是一个双结构域的激酶-GC信号分子，它可能在严重依赖第二信使cGMP的生物应激反应中发挥作用。
Second messengers have a key role in linking environmental stimuli to physiological responses. One such messenger, guanosine 3′,5′-cyclic monophosphate (cGMP), has long been known to be an essential signaling molecule in many different physiological processes in higher plants, including biotic stress responses. To date, however, the guanylyl cyclase (GC) enzymes that catalyze the formation of cGMP from GTP have largely remained elusive in higher plants. We have identified an Arabidopsis receptor type wall associated kinase–like molecule (AtWAKL10) as a candidate GC and provide experimental evidence to show that the intracellular domain of AtWAKL10431–700 can generate cGMP in vitro. Further, we also demonstrate that the molecule has kinase activity indicating that AtWAKL10 is a twin-domain catalytic protein. A co-expression and stimulus-specific expression analysis revealed that AtWAKL10 is consistently co-expressed with well characterized pathogen defense related genes and along with these genes is induced early and sharply in response to a range of pathogens and their elicitors. We demonstrate that AtWAKL10 is a twin-domain, kinase-GC signaling molecule that may function in biotic stress responses that are critically dependent on the second messenger cGMP.
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影响因子：
11.4
作者：
Eulgem, T;Rushton, PJ;Somssich, IE
通讯作者：
Somssich, IE
DOI：
10.1073/pnas.95.17.10328
发表时间：
1998-08-18
影响因子：
11.1
作者：
Durner, J;Wendehenne, D;Klessig, DF
通讯作者：
Klessig, DF
影响因子：
5.8
作者：
Al-Shahrour, F;Díaz-Uriarte, R;Dopazo, J
通讯作者：
Dopazo, J
影响因子：
7.2
作者：
He, ZH;He, DZ;Kohorn, BD
通讯作者：
Kohorn, BD
影响因子：
4.9
作者：
Decreux, A;Messiaen, J
通讯作者：
Messiaen, J