Exosome-functionalized polyetheretherketone-based implant with immunomodulatory property for enhancing osseointegration.
Exosome-functionalized polyetheretherketone-based implant with immunomodulatory property for enhancing osseointegration.
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外泌体功能化的基于聚醚醚酮的植入物具有免疫调节特性，可增强骨整合。
DOI：
10.1016/j.bioactmat.2021.02.005
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发表时间：
2021-09
影响因子：
18.9
通讯作者：
Ning C
中科院分区：
文献类型：
--
作者：
Fan L;Guan P;Xiao C;Wen H;Wang Q;Liu C;Luo Y;Ma L;Tan G;Yu P;Zhou L;Ning C
宿主免疫反应对生物材料的影响是决定种植体命运和骨再生性能的关键。骨髓干细胞来源的外泌体（exosomes，Exos）含有多种生物信号分子，具有免疫调节功能。在此，我们开发了一种BMSC衍生的Exos功能化植入物，以通过免疫调节加速骨整合。通过单宁酸（TA）与生物大分子的强相互作用，将BMSC衍生的Exos可逆地嵌入到TA修饰的磺化聚醚醚酮（SPEEK）上。SPEEK缓释的Exos可被共培养细胞吞噬，有效提高SPEEK的生物相容性。体外实验结果表明，负载Exos的SPEEK通过NF-κB通路促进巨噬细胞M2极化，从而促进BMSCs向成骨细胞分化。加载Exos的SPEEK的进一步体内大鼠气囊模型和大鼠股骨钻孔模型评估显示有效的巨噬细胞M2极化、期望的新骨形成和令人满意的骨整合。因此，BMSC衍生的Exos功能化植入物发挥骨免疫调节作用以促进成骨。植入的生物材料作为外来物质会引发一系列免疫反应。BMSC来源的具有免疫调节作用的exosomes适合作为生物活性涂层发挥骨免疫调节作用。外泌体功能化SPEEK可通过NF-κB途径调节巨噬细胞M2极化。具有骨免疫调节特性的外泌体功能化SPEEK可以促进骨整合。
The host immune response effecting on biomaterials is critical to determine implant fates and bone regeneration property. Bone marrow stem cells (BMSCs) derived exosomes (Exos) contain multiple biosignal molecules and have been demonstrated to exhibit immunomodulatory functions. Herein, we develop a BMSC-derived Exos–functionalized implant to accelerate bone integration by immunoregulation. BMSC-derived Exos were reversibly incorporated on tannic acid (TA) modified sulfonated polyetheretherketone (SPEEK) via the strong interaction of TA with biomacromolecules. The slowly released Exos from SPEEK can be phagocytosed by co-cultured cells, which could efficiently improve the biocompatibilities of SPEEK. In vitro results showed the Exos loaded SPEEK promoted macrophage M2 polarization via the NF-κB pathway to enhance BMSCs osteogenic differentiation. Further in vivo rat air-pouch model and rat femoral drilling model assessment of Exos loaded SPEEK revealed efficient macrophage M2 polarization, desirable new bone formation, and satisfactory osseointegration. Thus, BMSC-derived Exos–functionalized implant exerted osteoimmunomodulation effect to promote osteogenesis. The implanted biomaterials as foreign substances trigger a cascade of immune responses. BMSC-derived exosomes with immunomodulatory were suitable for bioactive coating to exert osteoimmunomodulation effect. Exosome-functionalized SPEEK can modulate macrophages M2 polarization via NF‐κB pathway. Exosome-functionalized SPEEK with osteoimmunomodulation properties can promote osseointegration.
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具有免疫调节功能的锌改性磺化聚醚醚酮表面，用于指导细胞命运和骨再生
DOI：
10.1002/advs.201800749
发表时间：
2018-10
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
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作者：
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作者：
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影响因子：
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作者：
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影响因子：
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通讯作者：
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