Transcription-generated torsional stress destabilizes nucleosomes.
Transcription-generated torsional stress destabilizes nucleosomes.
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DOI：
10.1038/nsmb.2723
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发表时间：
2014-01
影响因子：
16.8
通讯作者：
Henikoff S
中科院分区：
文献类型：
--
作者：
Teves SS;Henikoff S
As RNA Polymerase II (Pol II) transcribes a gene, it encounters an array of well-ordered nucleosomes. How it traverses through this array in vivo remains unresolved. One model proposes that torsional stress generated during transcription destabilizes nucleosomes ahead of Pol II. Here, we describe a method for high resolution mapping of underwound DNA using next-generation sequencing, and show that torsion is correlated with gene expression in Drosophila melanogaster cells. Accumulation of torsional stress, through topoisomerase inhibition, results in increased. Pol II at transcription start sites. Whereas Topo I inhibition results in increased nascent RNA transcripts, Topo II inhibition shows little change. Despite the different effects on Pol II elongation, topoisomerase inhibition results in increased nucleosome turnover and salt solubility within gene bodies, suggesting that the elongation-independent effects of torsional stress on nucleosome dynamics contributes to the destabilization of nucleosomes.
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影响因子：
16.8
作者：
Kouzine, Fedor;Gupta, Ashutosh;Baranello, Laura;Wojtowicz, Damian;Ben-Aissa, Khadija;Liu, Juhong;Przytycka, Teresa M.;Levens, David
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Ekwall, Karl
DOI：
10.1073/pnas.90.24.11508
发表时间：
1993-12-15
影响因子：
11.1
作者：
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影响因子：
11.4
作者：
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通讯作者：
Ekwall, Karl
影响因子：
2.9
作者：
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通讯作者：
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