Delivery of mesenchymal stem cells in chitosan/collagen microbeads for orthopedic tissue repair.
Delivery of mesenchymal stem cells in chitosan/collagen microbeads for orthopedic tissue repair.
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DOI：
10.1159/000348359
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Stegemann JP
中科院分区：
文献类型：
--
作者：
Wang L;Rao RR;Stegemann JP
在生物材料中微囊化和递送干细胞是以微创方式修复受损组织的有前途的方法。适当的生物材料生态位可以保护嵌入的细胞免受宿主组织中的挑战性环境的影响，同时还可以引导干细胞向所需的谱系分化。在这项研究中，成人间充质干细胞（MSC）包埋在水凝胶微珠组成的壳聚糖和I型胶原蛋白使用乳化过程。以乙二醛和β-甘油磷酸作为物理和化学交联剂引发基质材料的共聚合。通过控制乳化条件可以改变微球的平均粒径和粒径分布。制备了直径为82±19 μ m至290±78 μm的球形微珠。活力染色显示MSC在包封过程中存活（>90%活力），并且在培养中在9天的时间内在基质内扩散。使用培养基补充剂诱导成骨分化表明，MSC随着培养时间的推移增加osterix和骨钙素的基因表达，并沉积钙矿物质。骨唾液蛋白和I型胶原基因表达不受影响。以实际相关的流速通过标准针递送微珠不会对细胞活力产生不利影响，并且微珠也可以容易地模制成用于递送的规定几何形状。这样的基于蛋白质的微珠可以通过允许祖细胞在既具有保护性又具有指导性的微环境中的微创递送而在整形外科组织再生中具有效用。
Microencapsulation and delivery of stem cells in biomaterials is a promising approach to repairing damaged tissue in a minimally invasive manner. An appropriate biomaterial niche can protect the embedded cells from the challenging environment in the host tissue, while also directing stem cell differentiation toward the desired lineage. In this study, adult human mesenchymal stem cells (MSC) were embedded in hydrogel microbeads consisting of chitosan and Type I collagen using an emulsification process. Glyoxal and β-glycerophosphate were used as physical and chemical crosslinkers to initiate co-polymerization of the matrix materials. The average size and size distribution of the microbeads could be varied by controlling the emulsification conditions. Spheroidal microbeads ranging in diameter from 82±19 to 290±78 μm were produced. Viability staining showed that MSC survived the encapsulation process (>90% viability), and spread inside the matrix over a period of 9 days in culture. Induced osteogenic differentiation using medium supplements showed that MSC increased gene expression of osterix and osteocalcin over time in culture, and also deposited calcium mineral. Bone sialoprotein and Type I collagen gene expression were not affected. Delivery of microbeads through standard needles at practically relevant flow rates did not adversely affect cell viability, and microbeads also could be easily molded into prescribed geometries for delivery. Such protein-based microbeads may have utility in orthopaedic tissue regeneration by allowing minimally invasive delivery of progenitor cells in microenvironments that are both protective and instructive.
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DOI：
10.1089/ten.teb.2009.0270
发表时间：
2009-09
期刊：
Tissue engineering. Part B, Reviews
影响因子：
--
作者：
Lund AW;Yener B;Stegemann JP;Plopper GE
通讯作者：
Plopper GE
影响因子：
3.8
作者：
Du, Yanan;Ghodousi, Majid;Qi, Hao;Haas, Nikhil;Xiao, Wenqian;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
DOI：
10.1073/pnas.0903269107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Kilian, Kristopher A.;Bugarija, Branimir;Mrksich, Milan
通讯作者：
Mrksich, Milan
影响因子：
14
作者：
Cheng, Hiu-Wa;Luk, Keith D. K.;Chan, Barbara P.
通讯作者：
Chan, Barbara P.
影响因子：
4.1
作者：
HUNTER, GK;GOLDBERG, HA
通讯作者：
GOLDBERG, HA