Sequential assembly of cell-laden hydrogel constructs to engineer vascular-like microchannels.
Sequential assembly of cell-laden hydrogel constructs to engineer vascular-like microchannels.
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DOI：
10.1002/bit.23102
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发表时间：
2011-07
影响因子：
3.8
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Du, Yanan;Ghodousi, Majid;Qi, Hao;Haas, Nikhil;Xiao, Wenqian;Khademhosseini, Ali
微尺度技术，如微流体系统，为构建用于组织工程或体外组织模型的仿生血管样结构提供了强大的工具。最近，模块化的方法已经成为有吸引力的方法，在组织工程，以实现精确控制的架构，通过使用微工程组件。在这里，我们顺序组装微工程水凝胶（微凝胶）到水凝胶结构与嵌入式网络的微通道。通过光刻法制造具有预定义内部微通道的微凝胶阵列，并组装成具有多级互连管腔的3D管状结构。在当前的设置中，微凝胶的顺序组装发生在双相反应器中，并且通过刷针以产生物理力和流体剪切来启动。我们优化了组装条件，并成功地通过互连结构灌注流体。顺序组装过程不会显着影响微凝胶内的细胞活力，这表明其作为生物制造方法的前景。最后，为了尝试构建仿生3D血管系统，我们将内皮细胞和平滑肌细胞整合到具有同心微凝胶设计的组装结构中。该方法简单、快速、成本低，可用于构建具有仿生血管结构和其他复杂结构的组织结构。
Microscale technologies, such as microfluidic systems, provide powerful tools for building biomimetic vascular-like structures for tissue engineering or in vitro tissue models. Recently, modular approaches have emerged as attractive approaches in tissue engineering to achieve precisely controlled architectures by using microengineered components. Here, we sequentially assembled microengineered hydrogels (microgels) into hydrogel constructs with an embedded network of microchannels. Arrays of microgels with predefined internal microchannels were fabricated by photolithography and assembled into 3D tubular construct with multi-level interconnected lumens. In the current setting, the sequential assembly of microgels occurred in a biphasic reactor and was initiated by swiping a needle to generate physical forces and fluidic shear. We optimized the conditions for assembly and successfully perfused fluids through the interconnected constructs. The sequential assembly process does not significantly influence cell viability within the microgels indicating its promise as a biofabrication method. Finally, in an attempt to build a biomimetic 3D vasculature, we incorporated endothelial cells and smooth muscle cells into an assembled construct with a concentric microgel design. The sequential assembly is simple, rapid, cost-effective and could be used for fabricating tissue constructs with biomimetic vasculature and other complex architectures.
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影响因子：
14
作者：
Nichol, Jason W.;Koshy, Sandeep T.;Bae, Hojae;Hwang, Chang M.;Yamanlar, Seda;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
--
作者：
van der Meer AD;Poot AA;Duits MH;Feijen J;Vermes I
通讯作者：
Vermes I
影响因子：
3.4
作者：
Nichol JW;Khademhosseini A
通讯作者：
Khademhosseini A
DOI：
10.1073/pnas.0801866105
发表时间：
2008-07-15
影响因子：
11.1
作者：
Du, Yanan;Lo, Edward;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
6.1
作者：
Ling, Yibo;Rubin, Jamie;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali