Optical trapping assisted label-free and amplification-free detection of SARS-CoV-2 RNAs with an optofluidic nanopore sensor.
Optical trapping assisted label-free and amplification-free detection of SARS-CoV-2 RNAs with an optofluidic nanopore sensor.
复制标题
光阱辅助光流体纳米孔传感器检测SARS-CoV-2 rna的无标记和无扩增
DOI：
10.1016/j.bios.2021.113588
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发表时间：
2021-12-15
影响因子：
12.6
通讯作者：
Schmidt H
中科院分区：
文献类型：
--
作者：
Sampad MJN;Zhang H;Yuzvinsky TD;Stott MA;Hawkins AR;Schmidt H
用于分子生物标志物的超灵敏、多功能传感器是疾病诊断和个性化医疗的关键组成部分，正如COVID-19大流行所揭示的那样。集成的电纳米孔传感器可以通过无标记、直接检测单个生物分子来满足这一需求，但用于临床样品分析的全功能设备尚未开发。在这里，我们报告的扩增免费检测SARS-CoV-2 RNA的单分子灵敏度从临床鼻咽拭子样本上的电光流控芯片。该装置依赖于光学辅助递送的目标携带微珠的纳米孔的单一RNA检测后释放。证明了超过2，000倍的感测速率增强，并具有朝向较低浓度的有利缩放。靶特异性、芯片级集成和快速检测的结合确保了这种方法在104-109拷贝/mL的整个临床相关浓度范围内用于COVID-19诊断的实用性。
Ultrasensitive, versatile sensors for molecular biomarkers are a critical component of disease diagnostics and personalized medicine as the COVID-19 pandemic has revealed in dramatic fashion. Integrated electrical nanopore sensors can fill this need via label-free, direct detection of individual biomolecules, but a fully functional device for clinical sample analysis has yet to be developed. Here, we report amplification-free detection of SARS-CoV-2 RNAs with single molecule sensitivity from clinical nasopharyngeal swab samples on an electro-optofluidic chip. The device relies on optically assisted delivery of target carrying microbeads to the nanopore for single RNA detection after release. A sensing rate enhancement of over 2,000x with favorable scaling towards lower concentrations is demonstrated. The combination of target specificity, chip-scale integration and rapid detection ensures the practicality of this approach for COVID-19 diagnosis over the entire clinically relevant concentration range from 104-109 copies/mL.
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影响因子：
9.4
作者：
Kuypers, Jane;Wright, Nancy;Morrow, Rhoda
通讯作者：
Morrow, Rhoda
影响因子：
3.2
作者：
Parks, J. W.;Olson, M. A.;Schmidt, H.
通讯作者：
Schmidt, H.
影响因子：
10.8
作者：
Fologea, D;Gershow, M;Li, JL
通讯作者：
Li, JL
影响因子：
17.1
作者：
Shasha C;Henley RY;Stoloff DH;Rynearson KD;Hermann T;Wanunu M
通讯作者：
Wanunu M
DOI：
10.1109/lpt.2010.2051145
发表时间：
2010-07-12
期刊：
IEEE photonics technology letters : a publication of the IEEE Laser and Electro-optics Society
影响因子：
--
作者：
Lunt EJ;Wu B;Keeley JM;Measor P;Schmidt H;Hawkins AR
通讯作者：
Hawkins AR