Single serotonergic neurons that modulate aggression in Drosophila.
Single serotonergic neurons that modulate aggression in Drosophila.
复制标题
调节果蝇攻击性的单血清素能神经元。
DOI：
10.1016/j.cub.2014.09.051
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发表时间：
2014-11-17
期刊：
影响因子：
--
通讯作者：
Kravitz EA
中科院分区：
文献类型：
--
作者：
Alekseyenko OV;Chan YB;Fernandez MP;Bülow T;Pankratz MJ;Kravitz EA
单胺5-羟色胺（5 HT）多年来一直与各种物种的攻击性有关。然而，由于单胺能神经元也调节其他行为，因此控制攻击性的神经化学途径的阐述已经被证明是困难的。果蝇神经系统中大约有100个多巴胺能神经元，它们影响睡眠、昼夜节律、记忆和求偶。在果蝇的攻击模型中，整个多巴胺能系统的急性关闭使果蝇的战斗减少，而5-HT神经元的诱导激活则促进了攻击。利用交叉遗传学，我们限制了可以重复操作的5 HT神经元的数量，以识别那些调节攻击性的神经元。尽管最近使用类似的方法来发现攻击调节多巴胺能和FruM阳性肽能神经元，但构成攻击控制回路的神经元的下游解剖靶点仍然知之甚少。在这里，我们确定了一对对称的多巴胺能PLP神经元，这是必要的适当升级的侵略。在雄性果蝇中，沉默这些神经元会减少，激活它们会增加攻击性。跨突触伴侣的GFP重建（GRASP）分析表明，5 HT-PLP神经元在大脑的两个不同解剖区域中与表达5 HT 1A受体的神经元形成接触。反过来，这些表达5 HT 1A受体的神经元的激活会导致攻击性的减少。因此，我们的研究表明，攻击性可能会被抑制，至少部分是通过5 HT 1A受体神经元的抑制性输入来控制的，这些神经元可以通过激活5 HT-PLP神经元来释放。
Monoamine serotonin (5HT) has been linked to aggression for many years across species. However, elaboration of the neurochemical pathways that govern aggression has proven difficult because monoaminergic neurons also regulate other behaviors. There are about 100 serotonergic neurons in the Drosophila nervous system and they influence sleep, circadian rhythms, memory and courtship. In the Drosophila model of aggression the acute shut down of the entire serotonergic system yields flies that fight less, while induced activation of 5HT neurons promotes aggression. Using intersectional genetics we restricted the population of 5HT neurons that can be reproducibly manipulated to identify those that modulate aggression. Although similar approaches were used recently to find aggression-modulating dopaminergic and FruM –positive peptidergic neurons, the downstream anatomical targets of the neurons that make up aggression-controlling circuits remain poorly understood. Here we identified a symmetrical pair of serotonergic PLP neurons that are necessary for the proper escalation of aggression. Silencing these neurons reduced, and activating them increased aggression in male flies. GFP reconstitution across synaptic partners (GRASP) analyses suggests that 5HT-PLP neurons form contacts with 5HT1A receptor - expressing neurons in two distinct anatomical regions of the brain. Activation of these 5HT1A receptor-expressing neurons, in turn, caused reductions in aggression. Our studies, therefore, suggest that aggression may be held in check, at least in part, by inhibitory input from 5HT1A receptor-bearing neurons, which can be released by activation of the 5HT-PLP neurons.
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影响因子：
3.7
作者：
Becnel J;Johnson O;Luo J;Nässel DR;Nichols CD
通讯作者：
Nichols CD
影响因子：
64.5
作者：
Asahina K;Watanabe K;Duistermars BJ;Hoopfer E;González CR;Eyjólfsdóttir EA;Perona P;Anderson DJ
通讯作者：
Anderson DJ
影响因子：
3.4
作者：
Garcia-Garcia, Alvaro L.;Newman-Tancredi, Adrian;Leonardo, E. David
通讯作者：
Leonardo, E. David
影响因子：
16.2
作者：
Pool AH;Kvello P;Mann K;Cheung SK;Gordon MD;Wang L;Scott K
通讯作者：
Scott K
DOI：
10.1073/pnas.082102599
发表时间：
2002-04-16
影响因子：
11.1
作者：
Chen, S;Lee, AY;Kravitz, EA
通讯作者：
Kravitz, EA