Prevalence and characterization of human mecC methicillin-resistant Staphylococcus aureus isolates in England.
Prevalence and characterization of human mecC methicillin-resistant Staphylococcus aureus isolates in England.
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DOI：
10.1093/jac/dkt462
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发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Holmes MA
中科院分区：
文献类型：
--
作者：
Paterson GK;Morgan FJ;Harrison EM;Cartwright EJ;Török ME;Zadoks RN;Parkhill J;Peacock SJ;Holmes MA
关于编码最近描述的 mecA 同源物 mecC 的人群中耐甲氧西林金黄色葡萄球菌 (MRSA) 的流行病学和患病率的数据有限。为了解决这一知识差距，我们在英国进行了一项前瞻性流行率研究，以确定 MRSA 分离株中 mecC 的流行率。 2011-12 年间，从英格兰的六个临床微生物实验室中分别收集了来自个体患者的 335 份连续 MRSA 分离株。通过 PCR 或基因组测序对它们进行测试，以区分编码 mecA 和 mecC 的基因。 mecC 阳性分离株通过多位点序列分型、spa 分型、抗菌药物敏感性谱和使用市售试剂盒检测 PBP2a 进行了进一步表征。 2010 年检测的 MRSA 分离株中有 9 个呈 mecC 阳性，表明英格兰 MRSA 的流行率为 0.45%（95% CI 0.24%–0.85%）。其余的均为 mecA 阳性。这 9 个 mecC MRSA 分离株中的 8 个属于克隆复合体 130，另一个属于序列类型 425。这些 mecC MRSA 分离株对非 β-内酰胺抗生素的耐药性很少见，并且根据 BSAC 纸片扩散方法，使用苯唑西林和头孢西丁，所有分离株均被表型鉴定为 MRSA。然而，使用三种不同的商业 PBP2a 检测方法，所有九种 mecC 分离株均得出阴性结果。目前，mecC MRSA 在英格兰从人类中分离出的 MRSA 中很少见，这项研究为监测未来的变化提供了重要的基线患病率，鉴于丹麦报告的 mecC MRSA 患病率不断增加，这一点可能很重要。
There are limited data available on the epidemiology and prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in the human population that encode the recently described mecA homologue, mecC. To address this knowledge gap we undertook a prospective prevalence study in England to determine the prevalence of mecC among MRSA isolates. Three hundred and thirty-five sequential MRSA isolates from individual patients were collected from each of six clinical microbiology laboratories in England during 2011–12. These were tested by PCR or genome sequencing to differentiate those encoding mecA and mecC. mecC-positive isolates were further characterized by multilocus sequence typing, spa typing, antimicrobial susceptibility profile and detection of PBP2a using commercially available kits. Nine out of the 2010 MRSA isolates tested were mecC positive, indicating a prevalence among MRSA in England of 0.45% (95% CI 0.24%–0.85%). The remainder were mecA positive. Eight out of these nine mecC MRSA isolates belonged to clonal complex 130, the other being sequence type 425. Resistance to non-β-lactam antibiotics was rare among these mecC MRSA isolates and all were phenotypically identified as MRSA using oxacillin and cefoxitin according to BSAC disc diffusion methodology. However, all nine mecC isolates gave a negative result using three different commercial PBP2a detection assays. mecC MRSA are currently rare among MRSA isolated from humans in England and this study provides an important baseline prevalence rate to monitor future changes, which may be important given the increasing prevalence of mecC MRSA reported in Denmark.
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影响因子：
56.3
作者：
Garcia-Alvarez, Laura;Holden, Matthew T. G.;Lindsay, Heather;Webb, Cerian R.;Brown, Derek F. J.;Curran, Martin D.;Walpole, Enid;Brooks, Karen;Pickard, Derek J.;Teale, Christopher;Parkhill, Julian;Bentley, Stephen D.;Edwards, Giles F.;Girvan, E. Kirsty;Kearns, Angela M.;Pichon, Bruno;Hill, Robert L. R.;Larsen, Anders Rhod;Skov, Robert L.;Peacock, Sharon J.;Maskell, Duncan J.;Holmes, Mark A.
通讯作者：
Holmes, Mark A.
影响因子：
5.2
作者：
Skov, Robert;Larsen, Anders Rhod;Hill, Robert
通讯作者：
Hill, Robert
影响因子：
3.7
作者：
Monecke, Stefan;Gavier-Widen, Dolores;Ehricht, Ralf
通讯作者：
Ehricht, Ralf
影响因子：
2.1
作者：
Gindonis V;Taponen S;Myllyniemi AL;Pyörälä S;Nykäsenoja S;Salmenlinna S;Lindholm L;Rantala M
通讯作者：
Rantala M
影响因子：
9.4
作者：
Cartwright, Edward J. P.;Paterson, Gavin K.;Toeroek, M. Estee
通讯作者：
Toeroek, M. Estee