Occurrence and characterization of methicillin-resistant staphylococci from bovine mastitis milk samples in Finland.
Occurrence and characterization of methicillin-resistant staphylococci from bovine mastitis milk samples in Finland.
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DOI：
10.1186/1751-0147-55-61
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发表时间：
2013-08-28
影响因子：
2.1
通讯作者：
Rantala M
中科院分区：
文献类型：
--
作者：
Gindonis V;Taponen S;Myllyniemi AL;Pyörälä S;Nykäsenoja S;Salmenlinna S;Lindholm L;Rantala M
耐甲氧西林葡萄球菌(MRS)在牛乳房炎中的分离率越来越高。本研究的目的是评估芬兰乳房炎乳样中MRS的出现情况，并用分子方法鉴定MRS分离株。耐甲氧西林金黄色葡萄球菌(MRSA)在芬兰牛乳房炎中是一种罕见的发现。135株金黄色葡萄球菌中只有2株(1.5%)的mec基因阳性。其中一个携带mecA，属于spa型t172、SCCmec IV型和ST375，另一个含有mecC，为spa型t3256和st130。MRSA ST375在芬兰的人类MRSA分离株中很常见，但这在该国牛的mecC MRSA中是第一次报告。在牛乳腺炎来源的凝固酶阴性葡萄球菌(CONS)中，甲氧西林耐药更为常见。在所研究的两个CONS标本中，分别有5.2%(17/324)和1.8%(2/110)的菌株mecA阳性。其中18株为耐甲氧西林表皮葡萄球菌(MRSE)，分为6个独立的PFGE聚类群。在该国不同地区检测到一种脉冲型，表明克隆性传播。MRSE多为IV型，1例为V型，4例为非分型。与人类葡萄球菌数据库的比较表明，牛MRSE菌株与人MRSE分离株没有密切的亲缘关系。在芬兰的牛乳房炎中，MRS的发生率很低，尤其是MRSA。大多数耐甲氧西林的牛假单胞菌都是MRSE，我们发现了一种可能克隆传播的牛MRSE菌株的证据。这是芬兰牛源MRSAmecC ST130分离株的首次报道。这项研究为进一步监测MRS提供了一个基线。
Methicillin-resistant staphylococci (MRS) are increasingly being isolated in bovine mastitis. The aim of our study was to evaluate the occurrence of MRS in Finnish mastitis milk samples and characterize the MRS isolates using molecular methods. Methicillin-resistant S. aureus (MRSA) was a rare finding in bovine mastitis in Finland. Only two out of 135 (1.5%) S. aureus isolates were positive for mec genes. One of these carried mecA and was of spa type t172, SCCmec type IV and ST375, and the other harboured mecC, being spa type t3256, and ST130. MRSA ST375 is common among human MRSA isolates in Finland, but this is the first report in the country of bovine mecC MRSA. In coagulase-negative staphylococci (CoNS) originating from bovine mastitis, methicillin resistance was more common. In the two CoNS collections studied, 5.2% (17/324) and 1.8% (2/110) of the isolates were mecA positive. Eighteen of these were methicillin-resistant S. epidermidis (MRSE), which were divided into 6 separate PFGE clusters. One pulsotype was detected in different parts of the country, indicating clonal spread. Most MRSE (13/18) were of SCCmec type IV, one was of type V and four were non-typeable. Comparison with a human staphylococcal database indicated that bovine MRSE strains were not closely related to human MRSE isolates. The occurrence of MRS, especially MRSA, in bovine mastitis in Finland was low. Most methicillin-resistant bovine CoNS are MRSE, and we found evidence of a bovine MRSE strain that may spread clonally. This is the first report of a Finnish bovine isolate of MRSAmecC ST130. The study provides a baseline for further MRS monitoring.
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影响因子：
56.3
作者：
Garcia-Alvarez, Laura;Holden, Matthew T. G.;Lindsay, Heather;Webb, Cerian R.;Brown, Derek F. J.;Curran, Martin D.;Walpole, Enid;Brooks, Karen;Pickard, Derek J.;Teale, Christopher;Parkhill, Julian;Bentley, Stephen D.;Edwards, Giles F.;Girvan, E. Kirsty;Kearns, Angela M.;Pichon, Bruno;Hill, Robert L. R.;Larsen, Anders Rhod;Skov, Robert L.;Peacock, Sharon J.;Maskell, Duncan J.;Holmes, Mark A.
通讯作者：
Holmes, Mark A.
影响因子：
14.2
作者：
Ibrahem, S.;Salmenlinna, S.;Vuopio-Varkila, J.
通讯作者：
Vuopio-Varkila, J.
影响因子：
9.4
作者：
Hata, Eiji;Katsuda, Ken;Eguchi, Masashi
通讯作者：
Eguchi, Masashi
影响因子：
3.5
作者：
Moon, J. -S.;Lee, A. -R.;Koo, H. C.
通讯作者：
Koo, H. C.
影响因子：
5.2
作者：
Fessler, Andrea;Scott, Carly;Schwarz, Stefan
通讯作者：
Schwarz, Stefan