High-Fat Diet Modulates the Excitability of Neurons within the Brain-Liver Pathway.
High-Fat Diet Modulates the Excitability of Neurons within the Brain-Liver Pathway.
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DOI：
10.3390/cells12081194
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发表时间：
2023-04-20
期刊：
影响因子：
6
通讯作者：
Zsombok, Andrea
中科院分区：
文献类型：
--
作者：
Molinas, Adrien J. R.;Desmoulins, Lucie D. D.;Davis, Roslyn K. K.;Gao, Hong;Satou, Ryousuke;Derbenev, Andrei V. V.;Zsombok, Andrea
关键词：
肝交感神经的刺激增加葡萄糖产生和糖原分解。下丘脑室旁核（PVN）和延髓腹外侧和腹内侧（VLM/VMM）的交感前神经元的活动对交感神经输出有重要影响。交感神经系统（SNS）的活动增加在代谢性疾病的发展和进展中起着重要作用;然而，尽管中央回路的重要性，前交感肝相关神经元的兴奋性仍有待确定。在这里，我们测试的假设，在PVN和VLM/VMM的肝脏相关的神经元的活动是改变饮食诱导的肥胖小鼠，以及他们对胰岛素的反应。膜片钳记录从肝相关的PVN神经元，VLM投射PVN神经元，和腹侧脑干的前交感肝相关神经元。我们的数据表明，与对照饮食喂养的小鼠相比，高脂饮食（HFD）喂养的小鼠的肝脏相关PVN神经元的兴奋性增加。在肝脏相关神经元中检测到胰岛素受体表达，并且胰岛素抑制HFD小鼠中肝脏相关PVN和交感神经前VLM/VMM神经元的放电活动;然而，它不影响VLM投射的肝脏相关PVN神经元。这些发现进一步表明HFD改变了前自主神经元的兴奋性以及它们对胰岛素的反应。
Stimulation of hepatic sympathetic nerves increases glucose production and glycogenolysis. Activity of pre-sympathetic neurons in the paraventricular nucleus (PVN) of the hypothalamus and in the ventrolateral and ventromedial medulla (VLM/VMM) largely influence the sympathetic output. Increased activity of the sympathetic nervous system (SNS) plays a role in the development and progression of metabolic diseases; however, despite the importance of the central circuits, the excitability of pre-sympathetic liver-related neurons remains to be determined. Here, we tested the hypothesis that the activity of liver-related neurons in the PVN and VLM/VMM is altered in diet-induced obese mice, as well as their response to insulin. Patch-clamp recordings were conducted from liver-related PVN neurons, VLM-projecting PVN neurons, and pre-sympathetic liver-related neurons in the ventral brainstem. Our data demonstrate that the excitability of liver-related PVN neurons increased in high-fat diet (HFD)-fed mice compared to mice fed with control diet. Insulin receptor expression was detected in a population of liver-related neurons, and insulin suppressed the firing activity of liver-related PVN and pre-sympathetic VLM/VMM neurons in HFD mice; however, it did not affect VLM-projecting liver-related PVN neurons. These findings further suggest that HFD alters the excitability of pre-autonomic neurons as well as their response to insulin.
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影响因子：
5.8
作者：
Jensen KJ;Alpini G;Glaser S
通讯作者：
Glaser S
影响因子：
64.5
作者：
García-Cáceres C;Quarta C;Varela L;Gao Y;Gruber T;Legutko B;Jastroch M;Johansson P;Ninkovic J;Yi CX;Le Thuc O;Szigeti-Buck K;Cai W;Meyer CW;Pfluger PT;Fernandez AM;Luquet S;Woods SC;Torres-Alemán I;Kahn CR;Götz M;Horvath TL;Tschöp MH
通讯作者：
Tschöp MH
影响因子：
29
作者：
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通讯作者：
Luquet, Serge H.
影响因子：
8.3
作者：
Grassi, Guido;Biffi, Annalisa;Mancia, Giuseppe
通讯作者：
Mancia, Giuseppe
影响因子：
2.9
作者：
la Fleur, SE;Kalsbeek, A;Buijs, RM
通讯作者：
Buijs, RM