Organoid culture to study epithelial cell differentiation and barrier formation in the colon: bridging the gap between monolayer cell culture and human subject research.
Organoid culture to study epithelial cell differentiation and barrier formation in the colon: bridging the gap between monolayer cell culture and human subject research.
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在结肠中研究上皮细胞分化和屏障形成的器官培养：弥合单层细胞培养与人类学科研究之间的差距。
DOI：
10.1007/s11626-020-00534-6
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Aslam MN
中科院分区：
文献类型：
--
作者：
Varani J;McClintock SD;Aslam MN
类器官培养提供了一种强大的技术，可以弥合单层细胞培养与整个动物或人类受试者研究之间的差距。来自包括人类在内的多个物种的许多不同器官的组织已经成功地适应了类器官的生长。虽然还没有为所有组织类型建立最佳培养条件，但似乎大多数组织最终都适合这种培养。结肠是最早建立类器官培养技术的组织之一，也是最成功的组织之一。组织学上正常组织以及癌前组织和恶性组织（通常来自同一个体）的现成可用性使这成为可能。虽然在类器官培养中，个体肿瘤相对于其他肿瘤是高度可变的，但肿瘤组织与来自特定来源的组织学上正常的肿瘤组织之间存在高度的基因型一致性。此外，源材料和肿瘤组织在类器官培养中表现出高度的基因型一致性。即使在连续培养6-9个月后，突变谱的漂移也被证明是最小的。因此，在类器官培养中维持的结肠组织为原始组织提供了一个很好的替代物——然而，这个替代物与传统研究的细胞系一样，可以通过分子、药理学和免疫学方法进行干预。
Organoid culture provides a powerful technology that can bridge the gap between monolayer cell culture on the one hand and whole animal or human subject research on the other. Tissues from many different organs from multiple species, including human, have already been successfully adapted to organoid growth. While optimal culture conditions have not yet been established for all tissue types, it seems that most tissues will, ultimately, be amenable to this type of culture. The colon is one of the tissues in which organoid culture was first established as a technology and which has been most successfully employed. The ready availability of histologically normal tissue as well as both premalignant and malignant tissue (often from the same individual) makes this possible. While individual tumors are highly variable relative to one another in organoid culture, a high degree of genotypic consistency exists between the tumor tissue and the histologically normal counterpart from a given source. Further, source material and tumor tissue in organoid culture demonstrate a high degree of genotypic consistency. Even after 6–9 mo in continuous culture, drift in the mutational profile has been shown to be minimal. Colon tissue maintained in organoid culture, thus, provides a good surrogate for the tissue of origin—a surrogate, however, that is as amenable to intervention with molecular, pharmacological, and immunological approaches as are more-traditionally studied cell lines.
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影响因子：
3.7
作者：
Calderon-Gierszal EL;Prins GS
通讯作者：
Prins GS
DOI：
10.1016/j.jcf.2004.05.010
发表时间：
2004-08-01
期刊：
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
影响因子：
--
作者：
Bals, Robert;Beisswenger, Christoph;Chinet, Thierry
通讯作者：
Chinet, Thierry
影响因子：
7.2
作者：
Bootman, Martin D.;Bultynck, Geert
通讯作者：
Bultynck, Geert
影响因子：
7.7
作者：
Dye BR;Hill DR;Ferguson MA;Tsai YH;Nagy MS;Dyal R;Wells JM;Mayhew CN;Nattiv R;Klein OD;White ES;Deutsch GH;Spence JR
通讯作者：
Spence JR
影响因子：
18.4
作者：
Baker LA;Tiriac H;Clevers H;Tuveson DA
通讯作者：
Tuveson DA