Genetic diversity among major endemic strains of Leptospira interrogans in China.
Genetic diversity among major endemic strains of Leptospira interrogans in China.
复制标题
中国问号钩端螺旋体主要流行株遗传多样性
DOI：
10.1186/1471-2164-8-204
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发表时间：
2007-07-01
期刊：
影响因子：
4.4
通讯作者：
Guo XK
中科院分区：
文献类型：
--
作者：
He P;Sheng YY;Shi YZ;Jiang XG;Qin JH;Zhang ZM;Zhao GP;Guo XK
背景
钩端螺旋体病是一种世界性分布的人畜共患病。人类通过暴露于致病性钩端螺旋体而感染。污染的水或土壤。问号钩端螺旋体赖血清型和哥本哈根血清型基因组序列的可用性为鉴定不同致病性钩端螺旋体菌株之间的遗传多样性提供了机会。代表各种血清型（血清群和血清变型）的问号。
结果
比较基因组杂交（CGH）分析了L.问号赖型赖株与其它10株L.一个来自巴西的菌株是使用点样有3，528个Lai菌株的蛋白质编码序列（CDS）的微阵列鉴定的。通过比较Lai菌株和Copenhageni血清变型Fiocruz L1 - 130菌株的S/R杂交比率和计算机模拟序列相似性，设定用于检测一种供试菌株基因缺失的样品/参考（S/R）杂交临界比率。在检测的11个菌株中，发现275个CDS在至少一个菌株中缺失。L.问号线虫基因组中含有约2,917个CDS。编码基本细胞功能如翻译、能量产生和转换的基因是保守的。而菌株特异性基因包括那些编码与细胞表面结构或碳水化合物运输和代谢相关的蛋白质的基因。我们还发现了两个基因组岛（GI）在菌株赖含有基因分歧缺乏其他菌株。由于编码具有潜在致病功能的蛋白质的基因位于GI内，这些元件可能有助于疾病表现的变化。参与O-抗原生物合成的基因的差异也被确定为属于不同血清群的菌株，这为将来开发用于血清学分类的基因组分型工具提供了机会。
结论
中国流行的致病性钩端螺旋体株与钩端螺旋体的CGH分析。问号血清型赖菌株赖CDS斑点微阵列显示了2，917个共同的骨架CDS和菌株特异性基因，其编码的蛋白质主要与细胞表面结构和碳水化合物运输/代谢相关。在275个被认为至少有一个L。赖株的rfb基因簇和两个推测的基因组岛（GIA和B）。通过这项工作的菌株特异性基因检测将提供一个知识基础，为进一步调查的致病性L问号和/或开发有效的疫苗和/或诊断工具。
Background
Leptospirosis is a world-widely distributed zoonosis. Humans become infected via exposure to pathogenic Leptospira spp. from contaminated water or soil. The availability of genomic sequences of Leptospira interrogans serovar Lai and serovar Copenhageni opened up opportunities to identify genetic diversity among different pathogenic strains of L. interrogans representing various kinds of serotypes (serogroups and serovars).
Results
Comparative genomic hybridization (CGH) analysis was used to compare the gene content of L. interrogans serovar Lai strain Lai with that of other 10 L. interrogans strains prevailed in China and one identified from Brazil using a microarray spotted with 3,528 protein coding sequences (CDSs) of strain Lai. The cutoff ratio of sample/reference (S/R) hybridization for detecting the absence of genes from one tested strain was set by comparing the ratio of S/R hybridization and the in silico sequence similarities of strain Lai and serovar Copenhageni strain Fiocruz L1-130. Among the 11 strains tested, 275 CDSs were found absent from at least one strain. The common backbone of the L. interrogans genome was estimated to contain about 2,917 CDSs. The genes encoding fundamental cellular functions such as translation, energy production and conversion were conserved. While strain-specific genes include those that encode proteins related to either cell surface structures or carbohydrate transport and metabolism. We also found two genomic islands (GIs) in strain Lai containing genes divergently absent in other strains. Because genes encoding proteins with potential pathogenic functions are located within GIs, these elements might contribute to the variations in disease manifestation. Differences in genes involved in O-antigen biosynthesis were also identified for strains belonging to different serogroups, which offers an opportunity for future development of genomic typing tools for serological classification.
Conclusion
CGH analyses for pathogenic leptospiral strains prevailed in China against the L. interrogans serovar Lai strain Lai CDS-spotted microarrays revealed 2,917 common backbone CDSs and strain specific genes encoding proteins mainly related to cell surface structures and carbohydrated transport/metabolism. Of the 275 CDSs considered absent from at least one of the L. interrogans strains tested, most of them were clustered in the rfb gene cluster and two putative genomic islands (GI A and B) in strain Lai. The strain-specific genes detected via this work will provide a knowledge base for further investigating the pathogenesis of L interrogans and/or for the development of effective vaccines and/or diagnostic tools.
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