Systematic analysis of the maize cyclophilin gene family reveals ZmCYP15 involved in abiotic stress response
Systematic analysis of the maize cyclophilin gene family reveals ZmCYP15 involved in abiotic stress response
复制标题
玉米亲环蛋白基因家族的系统分析揭示ZmCYP15参与非生物胁迫反应
DOI：
10.1007/s11240-016-1132-0
复制
发表时间：
2017-03
影响因子：
3
通讯作者：
Li Xiaoyu
中科院分区：
文献类型：
--
作者：
Wang Qianqian;Wang Yu;Chai Wenbo;Song Nannan;Wang Jing;Cao Limin;Jiang Haiyang;Li Xiaoyu
亲环素(Cyclopilins，CyP)属于免疫亲和素超家族，具有肽基-Pro-顺式/反式异构酶活性，可催化Pro残基的顺式/反式异构化过程。以前的研究已经表明它们在植物中的重要性，但还没有关于玉米CYP家族的全面分析的报道。本研究对玉米CYP家族进行了全基因组分析，从玉米基因组中鉴定出39个ZmCYP基因(ZmCYP1~ZmCYP39)，这些基因在玉米10条染色体上分布不均。系统发育分析表明，这些ZmCYP基因之间存在较弱的亲缘关系。此外，它们的基因结构和基序模式在基因家族中也表现出变异。从39个ZmCYP基因中分别发现了4个节段性重复基因和1个串联重复基因对，这表明它们在玉米CYP家族的扩大中发挥了作用。对39个ZmCYP基因在玉米组织中的表达分析表明，它们的组织特异性表达模式不同。对筛选出的19个ZmCYP基因在盐胁迫下进行了实时定量聚合酶链式反应(RT-PCR)分析，揭示了它们在胁迫诱导下的表达谱。ZmCYP15在大肠杆菌中的异源表达增强了对非生物胁迫的耐受性。亚细胞定位分析表明，ZmCYP15定位于胞核和胞浆。本研究阐述了玉米CYP基因家族在逆境反应中的重要作用，为今后玉米遗传改良的研究和应用提供了参考。
Cyclophilins (CYPs) belong to the immunophilin superfamily, having the peptidyl prolyl cis/trans isomerase activity that can catalyze the cis/trans isomerisation process of proline residues. Previous studies have shown their importance in plants, but no comprehensive analysis of maize CYP family has been reported. In the present study, a whole-genome-wide analysis of maize CYP family was performed and 39 ZmCYP genes (ZmCYP1 to ZmCYP39) were identified from maize genome, which were unequally distributed on maize ten chromosomes. Phylogenetic analysis revealed a weak relationship among these ZmCYP genes. Furthermore, their gene structure and motif patterns also displayed variant within the gene family. Four segmental and one tandem duplicated gene pairs were found from 39 ZmCYP genes, respectively, indicating their roles in the expansion of maize CYP family. Expression analysis of 39 ZmCYP genes in maize tissues showed their differential tissue specific expression patterns. Quantitative real-time PCR analysis of 19 selected ZmCYP genes under salinity stress indicated their stress-inducible expression profile. Heterologous expression of ZmCYP15 in E. coli enhanced tolerance against abiotic stress. Subcellular localization analysis indicated ZmCYP15 was located in nucleus and cytoplasm. Our study describes the importance of the maize CYP gene family in stress response, and provides a reference for future study and application for maize genetic improvement.
登录
查看更多内容
影响因子：
7
作者：
Swigonov치, Z;Lai, JS;Messing, J
通讯作者：
Messing, J
影响因子：
3.7
作者：
Yadav NS;Singh VK;Singh D;Jha B
通讯作者：
Jha B
影响因子：
3.7
作者：
Agrawal C;Sen S;Yadav S;Rai S;Rai LC
通讯作者：
Rai LC
Plant-mPLoc：增强植物蛋白亚细胞定位预测能力的自上而下策略
影响因子：
3.7
作者：
Chou KC;Shen HB
通讯作者：
Shen HB
影响因子：
3.7
作者：
Zhao Y;Zhou Y;Jiang H;Li X;Gan D;Peng X;Zhu S;Cheng B
通讯作者：
Cheng B