A novel gene SbSI-2 encoding nuclear protein from a halophyte confers abiotic stress tolerance in E. coli and tobacco.
A novel gene SbSI-2 encoding nuclear protein from a halophyte confers abiotic stress tolerance in E. coli and tobacco.
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DOI：
10.1371/journal.pone.0101926
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Jha B
中科院分区：
文献类型：
--
作者：
Yadav NS;Singh VK;Singh D;Jha B
臂状盐角草是一种生长在沿海沼泽中的极端盐生植物。以前，我们已经报道了从盐角草中分离和鉴定的EST，其中包含大量新的/未知的盐响应基因序列。在本研究中，我们选择了一个新的盐诱导基因SBSI-2(Salicornia brachiata Salt-Inducble-2)进行功能鉴定。生物信息学分析表明，SBSI-2蛋白具有预测核定位信号和较强的蛋白质相互作用结构域。RFP：SbSI2融合蛋白的瞬时表达证实了SBSI-2是一种核定位蛋白。基因组组织学研究表明，SBSI-2在盐角藻基因组中是无内含子的，只有一个拷贝。定量RT-PCR分析表明，在盐胁迫和干燥条件下，SBSI-2的表达水平较高。将SBSI-2基因转化到大肠杆菌和烟草中进行功能鉴定。与单独载体相比，pET28a-SBSI-2重组大肠杆菌细胞具有更强的耐干性和耐盐性。与野生型相比，高表达SBSI-2的转基因烟草植株具有更好的生长参数、更高的相对含水量、更高的亲和性渗透调节物质积累、更低的Na+和ROS积累以及更低的电解质渗漏，从而提高了烟草的耐盐性和渗透性。在盐胁迫和渗透胁迫下，SBSI-2的过表达也提高了ROS清除基因和一些胁迫相关转录因子的转录水平。综上所述，这些结果表明SBSI-2可能在非生物胁迫耐受性中起着重要的正向调节作用。这表明SBSI-2是一种新的耐盐/渗透胁迫的决定因子，并表明它可能是一种潜在的作物耐非生物逆境基因的生物资源。
Salicornia brachiata is an extreme halophyte that grows luxuriantly in coastal marshes. Previously, we have reported isolation and characterization of ESTs from Salicornia with large number of novel/unknown salt-responsive gene sequences. In this study, we have selected a novel salt-inducible gene SbSI-2 (Salicornia brachiata salt-inducible-2) for functional characterization. Bioinformatics analysis revealed that SbSI-2 protein has predicted nuclear localization signals and a strong protein-protein interaction domain. Transient expression of the RFP:SbSI2 fusion protein confirmed that SbSI-2 is a nuclear-localized protein. Genomic organization study showed that SbSI-2 is intronless and has a single copy in Salicornia genome. Quantitative RT-PCR analysis revealed higher SbSI-2 expression under salt stress and desiccation conditions. The SbSI-2 gene was transformed in E. coli and tobacco for functional characterization. pET28a-SbSI-2 recombinant E. coli cells showed higher tolerance to desiccation and salinity compared to vector alone. Transgenic tobacco plants overexpressing SbSI-2 have improved salt- and osmotic tolerance, accompanied by better growth parameters, higher relative water content, elevated accumulation of compatible osmolytes, lower Na+ and ROS accumulation and lesser electrolyte leakage than the wild-type. Overexpression of the SbSI-2 also enhanced transcript levels of ROS-scavenging genes and some stress-related transcription factors under salt and osmotic stresses. Taken together, these results demonstrate that SbSI-2 might play an important positive modulation role in abiotic stress tolerance. This identifies SbSI-2 as a novel determinant of salt/osmotic tolerance and suggests that it could be a potential bioresource for engineering abiotic stress tolerance in crop plants.
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DOI：
10.1080/10425170601108555
发表时间：
2007-02-01
期刊：
DNA SEQUENCE
影响因子：
--
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
5.2
作者：
Cvikrova, Milena;Gemperlova, Lenka;Vankova, Radomira
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Vankova, Radomira
影响因子：
7.2
作者：
Hao, Yu-Jun;Wei, Wei;Chen, Shou-Yi
通讯作者：
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Wolf Psort：蛋白质定位预测因子。
影响因子：
14.9
作者：
Horton, Paul;Park, Keun-Joon;Obayashi, Takeshi;Fujita, Naoya;Harada, Hajime;Adams-Collier, C J;Nakai, Kenta
通讯作者：
Nakai, Kenta