Evolution and Diversity of the Antimicrobial Resistance Associated Mobilome in Streptococcus suis: A Probable Mobile Genetic Elements Reservoir for Other Streptococci.
Evolution and Diversity of the Antimicrobial Resistance Associated Mobilome in Streptococcus suis: A Probable Mobile Genetic Elements Reservoir for Other Streptococci.
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猪链球菌中与移动组相关的抗菌素耐药性的进化和多样性：其他链球菌可能的移动遗传元件库
DOI：
10.3389/fcimb.2016.00118
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发表时间：
2016
影响因子：
5.7
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Huang J;Ma J;Shang K;Hu X;Liang Y;Li D;Wu Z;Dai L;Chen L;Wang L
猪链球菌是一种以前被忽视的、新出现的多重耐药人畜共患病原体。移动遗传元件（MGE）在抗菌素耐药性（AMR）决定因素的种内和种间水平转移中发挥着关键作用。尽管之前的研究表明存在多种 MGE，但尚未对 AMR 相关移动组及其相互作用和进化进行全面分析。在这项研究中，我们提出了猪链球菌中 AMR 相关的移动组及其插入热点。整合接合元件 (ICE)、前噬菌体和串联 MGE 位于不同的插入位点，而 86% 的 AMR 相关 MGE 插入到 rplL 和 rum 位点。对四种致病性链球菌（无乳链球菌、肺炎链球菌、化脓性链球菌和猪链球菌）中 rplL 和 rum 位点插入的综合分析揭示了不同组 MGE 的存在，包括 Tn5252、ICESp1108 和 TnGBS2 组 ICE、Φm46.1 组原噬菌体、ICE_ICE 和ICE_prophage 串联 MGE。 ICESa2603家族的比较ICE基因组学揭示模块交换和获取/删除是MGE扩展和进化的主要机制。此外，串联 MGE 的观察反映了 MGE 多样性的一种新机制。此外，体外竞争试验表明，在不同的携带 MGE 的分离株之间没有观察到明显的适应性成本，并且接合试验揭示了 ICESa2603 家族的可转移性。我们的统计数据进一步表明，猪链球菌中 MGE 的流行率和多样性远高于其他三个物种，这促使我们假设猪链球菌可能是其他链球菌的 MGE 储存库。总之，我们的结果表明，MGE 的获得不仅赋予猪链球菌作为多药耐药病原体的能力，而且还代表了研究 MGE 的模块化进化和套娃的范例。
Streptococcus suis is a previously neglected, newly emerging multidrug-resistant zoonotic pathogen. Mobile genetic elements (MGEs) play a key role in intra- and interspecies horizontal transfer of antimicrobial resistance (AMR) determinants. Although, previous studies showed the presence of several MGEs, a comprehensive analysis of AMR-associated mobilome as well as their interaction and evolution has not been performed. In this study, we presented the AMR-associated mobilome and their insertion hotspots in S. suis. Integrative conjugative elements (ICEs), prophages and tandem MGEs were located at different insertion sites, while 86% of the AMR-associated MGEs were inserted at rplL and rum loci. Comprehensive analysis of insertions at rplL and rum loci among four pathogenic Streptococcus species (Streptococcus agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and S. suis) revealed the existence of different groups of MGEs, including Tn5252, ICESp1108, and TnGBS2 groups ICEs, Φm46.1 group prophage, ICE_ICE and ICE_prophage tandem MGEs. Comparative ICE genomics of ICESa2603 family revealed that module exchange and acquisition/deletion were the main mechanisms in MGEs' expansion and evolution. Furthermore, the observation of tandem MGEs reflected a novel mechanism for MGE diversity. Moreover, an in vitro competition assay showed no visible fitness cost was observed between different MGE-carrying isolates and a conjugation assay revealed the transferability of ICESa2603 family of ICEs. Our statistics further indicated that the prevalence and diversity of MGEs in S. suis is much greater than in other three species which prompted our hypothesis that S. suis is probably a MGEs reservoir for other streptococci. In conclusion, our results showed that acquisition of MGEs confers S. suis not only its capability as a multidrug resistance pathogen, but also represents a paradigm to study the modular evolution and matryoshkas of MGEs.
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影响因子：
4.5
作者：
Guglielmini J;Quintais L;Garcillán-Barcia MP;de la Cruz F;Rocha EP
通讯作者：
Rocha EP
影响因子：
3.7
作者：
Chen C;Tang J;Dong W;Wang C;Feng Y;Wang J;Zheng F;Pan X;Liu D;Li M;Song Y;Zhu X;Sun H;Feng T;Guo Z;Ju A;Ge J;Dong Y;Sun W;Jiang Y;Wang J;Yan J;Yang H;Wang X;Gao GF;Yang R;Wang J;Yu J
通讯作者：
Yu J
影响因子：
3.7
作者：
Beres SB;Musser JM
通讯作者：
Musser JM
影响因子：
4.9
作者：
Brenciani, Andrea;Tiberi, Erika;Giovanetti, Eleonora
通讯作者：
Giovanetti, Eleonora
影响因子：
11.3
作者：
Juhas M;van der Meer JR;Gaillard M;Harding RM;Hood DW;Crook DW
通讯作者：
Crook DW