Identification of two insecticide resistance markers in Ethiopian Anopheles stephensi mosquitoes using a multiplex amplicon sequencing assay.
Identification of two insecticide resistance markers in Ethiopian Anopheles stephensi mosquitoes using a multiplex amplicon sequencing assay.
复制标题
DOI：
10.1038/s41598-023-32336-7
复制
发表时间：
2023-04-05
影响因子：
4.6
通讯作者：
Campino, Susana
中科院分区：
文献类型：
--
作者：
Acford-Palmer, Holly;Phelan, Jody E.;Tadesse, Fitsum G.;Kristan, Mojca;Collins, Emma;Spadar, Anton;Walker, Thomas;Bousema, Teun;Messenger, Louisa A.;Clark, Taane G.;Campino, Susana
自2012年在吉布提首次发现斯氏按蚊以来，斯氏按蚊已在非洲之角入侵并定居，最近又入侵尼日利亚。这种病媒的扩大对控制和消灭疟疾的努力构成重大威胁。病媒综合管理是用于阻断疾病传播的主要战略;然而，杀虫剂抗药性的不断增加有可能逆转全球疟疾控制方面取得的成果。我们提出了一种新一代扩增子测序方法，用于高通量监测杀虫剂抗性基因（ace 1，GSTe 2，vgsc和rdl），物种鉴定和遗传多样性（its 2和cox 1）的表征。史蒂芬西九十五安。筛选了收集于埃塞俄比亚的stephensi蚊子，鉴定了104个SNP，包括敲低突变L958 F（家蝇中的L1014 F），以及首次在该载体物种中rdl基因座中的A296 S置换（黑腹果蝇中的A301 S）。还鉴定了另外两个氨基酸取代（ace 1-N177 D，GSTe 2-V189 L），但先前未涉及杀虫剂抗性。线粒体cox 1基因的遗传多样性揭示了埃塞俄比亚安。来自巴基斯坦、苏丹和吉布提的样本。总体而言，我们提出了一个可靠的，具有成本效益的策略，使用扩增子测序来监测已知的杀虫剂抗性突变，有可能识别新的遗传变异，以协助高通量监测杀虫剂抗性在安。stephensi种群
Since its first detection in 2012 in Djibouti, Anopheles stephensi has invaded and established in the Horn of Africa, and more recently Nigeria. The expansion of this vector poses a significant threat to malaria control and elimination efforts. Integrated vector management is the primary strategy used to interrupt disease transmission; however, growing insecticide resistance is threatening to reverse gains in global malaria control. We present a next-generation amplicon-sequencing approach, for high-throughput monitoring of insecticide resistance genes (ace1, GSTe2, vgsc and rdl), species identification and characterization of genetic diversity (its2 and cox1) in An. stephensi. Ninety-five An. stephensi mosquitoes, collected in Ethiopia, were screened, identifying 104 SNPs, including the knock-down mutation L958F (L1014F in Musca domestica), and for the first time in this vector species, the A296S substitution (A301S in Drosophila melanogaster) in the rdl locus. Two other amino acid substitutions (ace1-N177D, GSTe2-V189L) were also identified but have not been previously implicated in insecticide resistance. Genetic diversity in the mitochondrial cox1 gene revealed shared haplotypes between Ethiopian An. stephensi with samples from Pakistan, Sudan, and Djibouti. Overall, we present a reliable, cost-effective strategy using amplicon-sequencing to monitor known insecticide resistance mutations, with the potential to identify new genetic variants, to assist in the high-throughput surveillance of insecticide resistance in An. stephensi populations.
登录
查看更多内容
影响因子：
3.2
作者：
Carter TE;Yared S;Getachew D;Spear J;Choi SH;Samake JN;Mumba P;Dengela D;Yohannes G;Chibsa S;Murphy M;Dissanayake G;Flately C;Lopez K;Janies D;Zohdy S;Irish SR;Balkew M
通讯作者：
Balkew M
影响因子：
14.9
作者：
Letunic I;Bork P
通讯作者：
Bork P
影响因子：
10.7
作者：
Grau-Bové X;Tomlinson S;O'Reilly AO;Harding NJ;Miles A;Kwiatkowski D;Donnelly MJ;Weetman D;Anopheles gambiae 1000 Genomes Consortium
通讯作者：
Anopheles gambiae 1000 Genomes Consortium
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
7.7
作者：
Makunin A;Korlević P;Park N;Goodwin S;Waterhouse RM;von Wyschetzki K;Jacob CG;Davies R;Kwiatkowski D;St Laurent B;Ayala D;Lawniczak MKN
通讯作者：
Lawniczak MKN