Genetic diversity of Anopheles stephensi in Ethiopia provides insight into patterns of spread.
Genetic diversity of Anopheles stephensi in Ethiopia provides insight into patterns of spread.
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DOI：
10.1186/s13071-021-05097-3
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发表时间：
2021-12-11
影响因子：
3.2
通讯作者：
Balkew M
中科院分区：
文献类型：
--
作者：
Carter TE;Yared S;Getachew D;Spear J;Choi SH;Samake JN;Mumba P;Dengela D;Yohannes G;Chibsa S;Murphy M;Dissanayake G;Flately C;Lopez K;Janies D;Zohdy S;Irish SR;Balkew M
最近在非洲之角（HOA）发现南亚疟疾病媒斯氏按蚊（Anopheles stephensi）引起了人们对这种蚊子对该区域疟疾传播的影响的关注。分析An。斯氏体蚊的遗传多样性和种群结构可以为了解HOA地区斯氏体蚊的历史提供线索，以改进对未来传播的预测。本文研究了黄杨的遗传多样性。为了了解这种入侵种群的历史，检测表明其范围扩展到该地区。我们对188an的细胞色素氧化酶亚基I （COI）和细胞色素B基因（CytB）进行了测序。2018年在埃塞俄比亚的10个地点收集到了斯蒂芬斯氏菌。对埃塞俄比亚序列进行了群体遗传、系统发育和最小跨越网络分析。还使用通用脊椎动物CytB测序进行了血粉来源的分子鉴定。六十一年。在埃塞俄比亚东部的东南部位点（Kebridehar、Godey和Degehabur）中，东北部位点（Semera、Bati和Gewana镇）的stephensi - cytb单倍型数量最多。我们观察到种群分化，与中部遗址（Erer Gota、Dire Dawa和Awash Sebat Kilo）和东南部遗址相比，东北部遗址的种群分化程度最高。系统发育和网络分析表明，该系统具有较强的适应性。斯蒂芬氏蝇与安氏蝇在基因上更相似。而不是来自阿拉伯半岛。最后，分子血餐分析揭示了以牛、山羊、狗和人类为食的证据，以及多种（混合）血餐的证据。我们证明了An。斯蒂芬氏菌在埃塞俄比亚具有遗传多样性，并有地理结构的证据。多样性水平的变化支持了最近引入An的假设。斯蒂芬氏菌相对于东北部地区传入埃塞俄比亚东南部。我们也发现了支持HOA An假说的证据。斯蒂芬氏蝇种群起源于南亚而不是阿拉伯半岛。动物食性和人食性喂养的证据支持有必要进一步调查牲畜流动在该地区媒介传播中发挥作用的可能性。在线版本包含补充材料，可在10.1186/s13071-021-05097-3获得。
The recent detection of the South Asian malaria vector Anopheles stephensi in the Horn of Africa (HOA) raises concerns about the impact of this mosquito on malaria transmission in the region. Analysis of An. stephensi genetic diversity and population structure can provide insight into the history of the mosquito in the HOA to improve predictions of future spread. We investigated the genetic diversity of An. stephensi in eastern Ethiopia, where detection suggests a range expansion into this region, in order to understand the history of this invasive population. We sequenced the cytochrome oxidase subunit I (COI) and cytochrome B gene (CytB) in 187 An. stephensi collected from 10 sites in Ethiopia in 2018. Population genetic, phylogenetic, and minimum spanning network analyses were conducted for Ethiopian sequences. Molecular identification of blood meal sources was also performed using universal vertebrate CytB sequencing. Six An. stephensi COI-CytB haplotypes were observed, with the highest number of haplotypes in the northeastern sites (Semera, Bati, and Gewana towns) relative to the southeastern sites (Kebridehar, Godey, and Degehabur) in eastern Ethiopia. We observed population differentiation, with the highest differentiation between the northeastern sites compared to central sites (Erer Gota, Dire Dawa, and Awash Sebat Kilo) and the southeastern sites. Phylogenetic and network analysis revealed that the HOA An. stephensi are more genetically similar to An. stephensi from southern Asia than from the Arabian Peninsula. Finally, molecular blood meal analysis revealed evidence of feeding on cows, goats, dogs, and humans, as well as evidence of multiple (mixed) blood meals. We show that An. stephensi is genetically diverse in Ethiopia and with evidence of geographical structure. Variation in the level of diversity supports the hypothesis for a more recent introduction of An. stephensi into southeastern Ethiopia relative to the northeastern region. We also find evidence that supports the hypothesis that HOA An. stephensi populations originate from South Asia rather than the Arabian Peninsula. The evidence of both zoophagic and anthropophagic feeding support the need for additional investigation into the potential for livestock movement to play a role in vector spread in this region. The online version contains supplementary material available at 10.1186/s13071-021-05097-3.
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影响因子：
3.2
作者：
Balkew, Meshesha;Mumba, Peter;Carter, Tamar E.
通讯作者：
Carter, Tamar E.
影响因子：
64.8
作者：
Huestis, Diana L.;Dao, Adama;Lehmann, Tovi
通讯作者：
Lehmann, Tovi
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM
影响因子：
6.6
作者：
Leigh, Jessica W.;Bryant, David
通讯作者：
Bryant, David
影响因子：
5.8
作者：
Koressaar, Triinu;Remm, Maido
通讯作者：
Remm, Maido