Increased surfactant protein-D levels in the airways of preterm neonates with sepsis indicated responses to infectious challenges.
Increased surfactant protein-D levels in the airways of preterm neonates with sepsis indicated responses to infectious challenges.
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败血症的早产新生儿气道中的表面活性剂蛋白-D水平增加表明对感染性挑战的反应。
DOI：
10.1111/apa.14630
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发表时间：
2019-05
期刊：
影响因子：
--
通讯作者：
Todd DA
中科院分区：
文献类型：
--
作者：
Mackay RA;Townsend JP;Calvert J;Anthony M;Wilkinson AR;Postle AD;Clark HW;Todd DA
脓毒症是多因素的，对早产儿具有潜在的破坏性。感染过程中表面活性剂蛋白- D （SP - D）、磷脂酰胆碱（PC）和磷脂酰胆碱分子种类的变化可能提示先天免疫或败血症期间的炎症。我们的目的是比较这些重要的肺分子在通气新生儿没有或有败血症。从2000年10月至2002年3月在英国牛津约翰拉德克利夫医院新生儿重症监护室接受的23-35周出生的早产儿中收集气管内吸入物。在第1天至第30天收集样品，使用酶联免疫吸附法和质谱法分析SP - D、总PC和PC分子种浓度。我们发现8/54（14.8%）的新生儿发生败血症。与对照组相比，脓毒症期间SP - D （p < 0.0001）、单不饱和和双不饱和PC显著升高（p = 0.05），多不饱和PC显著降低（p < 0.01）。脓毒症期间SP‐D:PC比值显著升高（p < 0.001），脓毒症新生儿SP‐D浓度与胎龄直接相关（r2 = 0.389, p < 0.01）。脓毒症期间SP - D水平升高和PC分子种类的变化与直接或间接的肺部炎症过程一致。尽管出生时表面活性蛋白水平较低，但早产儿能够对感染挑战产生急性炎性先天免疫反应。
Sepsis is multifactorial and potentially devastating for preterm neonates. Changes in surfactant protein‐D (SP‐D), phosphatidylcholine (PC) and PC molecular species during infection may indicate innate immunity or inflammation during sepsis. We aimed to compare these important pulmonary molecules in ventilated neonates without or with sepsis. Endotracheal aspirates were collected from preterm neonates born at 23–35 weeks and admitted to the neonatal intensive care unit at the John Radcliffe Hospital, Oxford, UK, from October 2000 to March 2002. Samples were collected at one day to 30 days and analysed for SP‐D, total PC and PC molecular species concentrations using enzyme‐linked immunosorbent assay and mass spectrometry. We found that 8/54 (14.8%) neonates developed sepsis. SP‐D (p < 0.0001), mono‐ and di‐unsaturated PC were significantly increased (p = 0.05), and polyunsaturated PC was significantly decreased (p < 0.01) during sepsis compared to controls. SP‐D:PC ratios were significantly increased during sepsis (p < 0.001), and SP‐D concentrations were directly related to gestational age in neonates with sepsis (r2 = 0.389, p < 0.01). Increased SP‐D levels and changes in PC molecular species during sepsis were consistent with direct or indirect pulmonary inflammatory processes. Very preterm neonates we able to mount an acute inflammatory innate immune response to infectious challenges, despite low levels of surfactant proteins at birth.
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DOI：
10.1136/adc.67.4_spec_no.378
发表时间：
1992-04-01
影响因子：
4.4
作者：
ASHTON, MR;POSTLE, AD;NORMAND, ICS
通讯作者：
NORMAND, ICS
DOI：
10.1152/ajplung.1998.274.6.l958
发表时间：
1998-06-01
影响因子：
4.9
作者：
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通讯作者：
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影响因子：
3.6
作者：
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通讯作者：
Shaw, NJ
影响因子：
4.4
作者：
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通讯作者：
Holmskov, U
影响因子：
4.8
作者：
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