Telocytes in human liver fibrosis.
Telocytes in human liver fibrosis.
复制标题
人类肝纤维化中的特洛细胞
DOI：
10.1111/jcmm.12542
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发表时间：
2015-03
影响因子：
5.3
通讯作者：
Popescu LM
中科院分区：
文献类型：
--
作者：
Fu S;Wang F;Cao Y;Huang Q;Xiao J;Yang C;Popescu LM
肝纤维化是一种创伤愈合反应，其涉及多种细胞类型以包裹损伤。端细胞（Telocyte，TC）是一种新型的间质细胞，广泛存在于包括肝脏在内的多种组织器官中。据报道，TC在心肌梗死后的纤维化区域、由溃疡性结肠炎或克罗恩病引起的人类间质壁的纤维化重塑以及系统性硬化症的皮肤中减少。然而，TC在人类肝纤维化中的作用仍不清楚。收集来自人肝活检的肝样品。所有样品用Masson三色染色以确定纤维化。通过几种免疫荧光染色鉴定TC，包括CD 34和c-kit/CD 117或波形蛋白或PDGF受体-α或β的双标记。我们发现，在人类肝纤维化中，肝TC显著减少27%-60%，这表明TC的损失可能导致细胞外基质的组织改变，失去对成纤维细胞/肌成纤维细胞活性的控制，并有利于纤维化的发生。添加TC可能有助于开发针对人类肝纤维化的有效和靶向的抗纤维化疗法。
Liver fibrosis is a wound-healing response which engages a variety of cell types to encapsulate injury. Telocyte (TC), a novel type of interstitial cell, has been identified in a variety of tissues and organs including liver. TCs have been reported to be reduced in fibrotic areas after myocardial infarction, human interstitial wall's fibrotic remodelling caused either by ulcerative colitis or Crohn's disease, and skin of systemic sclerosis. However, the role of TCs in human liver fibrosis remains unclear. Liver samples from human liver biopsy were collected. All samples were stained with Masson's trichrome to determine fibrosis. TCs were identified by several immunofluorescence stainings including double labelling for CD34 and c-kit/CD117, or vimentin, or PDGF Receptor-α, or β. We found that hepatic TCs were significantly decreased by 27%–60% in human liver fibrosis, suggesting that loss of TCs might lead to the altered organization of extracellular matrix and loss the control of fibroblast/myofibroblast activity and favour the genesis of fibrosis. Adding TCs might help to develop effective and targeted antifibrotic therapies for human liver fibrosis.
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