Telocytes and stem cells in limbus and uvea of mouse eye.
Telocytes and stem cells in limbus and uvea of mouse eye.
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DOI：
10.1111/jcmm.12111
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发表时间：
2013-08
影响因子：
5.3
通讯作者：
Popescu LM
中科院分区：
文献类型：
--
作者：
Luesma MJ;Gherghiceanu M;Popescu LM
干细胞(SC)治疗眼科疾病的潜力是公认的。然而，结果仍然令人鼓舞，因为人们对眼睛再生、再生和/或修复的机制知之甚少。因此，了解干细胞/祖细胞在眼内所处的特定组织环境是至关重要的。一种新的间质细胞--端粒细胞(TC)(http://www.telocytes.com))是最近通过电子显微镜(EM)鉴定出来的。TCs有很长(几十微米)和很细(200 nm以下)的延长，称为端粒(TP)，形成嵌入SCs的异质细胞网络。在小鼠的巩膜、角膜缘和葡萄膜中，我们用电子断层扫描和电子显微镜观察到了TCS。此外，光镜下可见SCs位于虹膜和角膜缘的前层。在葡萄膜和巩膜中，粘附素和缝隙连接被发现连接着一个网络中的TC。TCS与其他细胞：干细胞、黑素细胞、神经末梢和巨噬细胞之间存在纳米接触(电子致密结构)。这些细胞间的‘脚’连接着细胞间的裂缝(大约10纳米宽)。此外，TCS还将胞外小体(直径达100 nm的胞外囊泡)输送到虹膜基质的其他细胞。TCS与SCs的超微结构纳米接触以及TCS旁分泌通过上皮和间质SC壁龛中的外体影响，提示TCS在眼再生中具有重要的作用。
The potential of stem cell (SC) therapies for eye diseases is well-recognized. However, the results remain only encouraging as little is known about the mechanisms responsible for eye renewal, regeneration and/or repair. Therefore, it is critical to gain knowledge about the specific tissue environment (niches) where the stem/progenitor cells reside in eye. A new type of interstitial cell–telocyte (TC) (http://www.telocytes.com) was recently identified by electron microscopy (EM). TCs have very long (tens of micrometres) and thin (below 200 nm) prolongations named telopodes (Tp) that form heterocellular networks in which SCs are embedded. We found TCs by EM and electron tomography in sclera, limbus and uvea of the mouse eye. Furthermore, EM showed that SCs were present in the anterior layer of the iris and limbus. Adhaerens and gap junctions were found to connect TCs within a network in uvea and sclera. Nanocontacts (electron-dense structures) were observed between TCs and other cells: SCs, melanocytes, nerve endings and macrophages. These intercellular ‘feet’ bridged the intercellular clefts (about 10 nm wide). Moreover, exosomes (extracellular vesicles with a diameter up to 100 nm) were delivered by TCs to other cells of the iris stroma. The ultrastructural nanocontacts of TCs with SCs and the TCs paracrine influence via exosomes in the epithelial and stromal SC niches suggest an important participation of TCs in eye regeneration.
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影响因子：
3.6
作者：
Hinescu, Mihail E.;Gherghiceanu, Mihaela;Suciu, Laura;Popescu, Laurentiu M.
通讯作者：
Popescu, Laurentiu M.
影响因子：
5.3
作者：
Cantarero Carmona I;Luesma Bartolomé MJ;Junquera Escribano C
通讯作者：
Junquera Escribano C
影响因子：
5.3
作者：
Ceafalan L;Gherghiceanu M;Popescu LM;Simionescu O
通讯作者：
Simionescu O
DOI：
10.1530/rep-12-0369
发表时间：
2013-04
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Cretoiu SM;Cretoiu D;Marin A;Radu BM;Popescu LM
通讯作者：
Popescu LM
影响因子：
2.7
作者：
Blenkinsop TA;Corneo B;Temple S;Stern JH
通讯作者：
Stern JH