Spindle shaped human mesenchymal stem/stromal cells from amniotic fluid promote neovascularization.
Spindle shaped human mesenchymal stem/stromal cells from amniotic fluid promote neovascularization.
复制标题
DOI：
10.1371/journal.pone.0054747
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Watt SM
中科院分区：
文献类型：
--
作者：
Roubelakis MG;Tsaknakis G;Pappa KI;Anagnou NP;Watt SM
在羊水穿刺术中获得的人羊水在培养时产生至少两种形态学上不同的间充质干细胞/基质细胞（MSC）亚群。其中，梭形羊水MSC（SS-AF-MSC）含有具有增强的成脂、成骨和成软骨能力的多能细胞。在这里，我们首次证明了这些SS-AF-MSC在体外和体内模型中通过脐带血（UCB）内皮集落形成细胞（ECFC）衍生细胞支持新血管形成的能力。有趣的是，尽管当将支持性SS-AF-MSC与骨髓MSC（BMSC）或人真皮成纤维细胞（hDF）的最佳血管生成支持性批次进行比较时，体外血管小管形成的动力学相似，但SS-AF-MSC比BMSC更有效地支持体内血管小管形成。在NOD/SCID小鼠中，人血管与鼠血管融合，证明其功能性。蛋白质组分析仪阵列分析揭示了SS-AF-MSC与hDF和BMSC相对的血管生成因子的共同和不同的分泌谱。因此，SS-AF-MSC被认为在发育上比成人BMSC更不成熟，并且在其潜力方面介于成人干细胞和胚胎干细胞之间，具有支持增加的新血管形成的额外且非常有趣的潜力，进一步增强了其作为组织修复和再生的载体的前景。
Human amniotic fluid obtained at amniocentesis, when cultured, generates at least two morphologically distinct mesenchymal stem/stromal cell (MSC) subsets. Of these, the spindle shaped amniotic fluid MSCs (SS-AF-MSCs) contain multipotent cells with enhanced adipogenic, osteogenic and chondrogenic capacity. Here, we demonstrate, for the first time, the capacity of these SS-AF-MSCs to support neovascularization by umbilical cord blood (UCB) endothelial colony forming cell (ECFC) derived cells in both in vitro and in vivo models. Interestingly, although the kinetics of vascular tubule formation in vitro were similar when the supporting SS-AF-MSCs were compared with the best vasculogenic supportive batches of bone marrow MSCs (BMSCs) or human dermal fibroblasts (hDFs), SS-AF-MSCs supported vascular tubule formation in vivo more effectively than BMSCs. In NOD/SCID mice, the human vessels inosculated with murine vessels demonstrating their functionality. Proteome profiler array analyses revealed both common and distinct secretion profiles of angiogenic factors by the SS-AF-MSCs as opposed to the hDFs and BMSCs. Thus, SS-AF-MSCs, which are considered to be less mature developmentally than adult BMSCs, and intermediate between adult and embryonic stem cells in their potentiality, have the additional and very interesting potential of supporting increased neovascularisation, further enhancing their promise as vehicles for tissue repair and regeneration.
登录
查看更多内容
产科因素和储存温度对脐带血形成细胞内皮菌落产量的影响。
影响因子：
9.8
作者：
Coldwell, Kate E.;Lee, Stephanie J.;Kean, Jennifer;Khoo, Cheen P.;Tsaknakis, Grigorios;Smythe, Jon;Watt, Suzanne M.
通讯作者：
Watt, Suzanne M.
DOI：
10.1111/j.1471-0528.1985.tb03049.x
发表时间：
1985-01-01
期刊：
BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY
影响因子：
--
作者：
LESCHOT, NJ;VERJAAL, M;TREFFERS, PE
通讯作者：
TREFFERS, PE
影响因子：
6.5
作者：
Erices, A;Conget, P;Minguell, JJ
通讯作者：
Minguell, JJ
影响因子：
4
作者：
Bitsika, Vasiliki;Roubelakis, Maria G.;Anagnou, Nicholas P.
通讯作者：
Anagnou, Nicholas P.
影响因子：
2.1
作者：
De Spiegelaere, Ward;Cornillie, Pieter;Bahramsoltani, Mahtab
通讯作者：
Bahramsoltani, Mahtab