Effects of obstetric factors and storage temperatures on the yield of endothelial colony forming cells from umbilical cord blood.
Effects of obstetric factors and storage temperatures on the yield of endothelial colony forming cells from umbilical cord blood.
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产科因素和储存温度对脐带血形成细胞内皮菌落产量的影响。
DOI：
10.1007/s10456-011-9222-4
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发表时间：
2011-09
期刊：
影响因子：
9.8
通讯作者：
Watt, Suzanne M.
中科院分区：
文献类型：
--
作者：
Coldwell, Kate E.;Lee, Stephanie J.;Kean, Jennifer;Khoo, Cheen P.;Tsaknakis, Grigorios;Smythe, Jon;Watt, Suzanne M.
关键词：
由于脐带血(UCB)是内皮细胞集落形成细胞(ECFC)的丰富来源，我们的目的有两个：(1)研究获得最高ECFC产量的潜在产科选择标准；(2)确定新鲜UCB的瞬时储存温度和UCB单位的冷冻保存是否影响ECFC产量和功能。根据ECFC的克隆增殖能力评价冻存前后ECFC的质量。在检查的20个因素中，胎盘重量是唯一具有统计学意义的产科因素，可以预测脐带血中ECFC的频率。对储存效果的研究表明，与22℃储存相比，新鲜UCB在4℃下瞬时储存会降低ECFC的得率，而UCB MNC的冷冻保存会显著降低ECFC的回收率。据我们所知，这是第一次证明胎盘重量和加工或培养前的储存温度对脐带血ECFC频率有显著影响。我们的研究进一步支持了UCB MNC冷冻保存影响ECFC恢复的证据。
As umbilical cord blood (UCB) is a rich source of endothelial colony-forming cells (ECFC), our aim was twofold: (1) to examine potential obstetric selection criteria for achieving the highest ECFC yields from UCB units, and (2) to determine whether transient storage temperatures of fresh UCB and cryopreservation of UCB units affected ECFC yield and function. ECFC quality was assessed before and after cryopreservation by their clonogenic proliferative potential. Of the 20 factors examined, placental weight was the only statistically significant obstetric factor that predicted ECFC frequency in UCB. Studies on the effects of storage revealed that transient storage of fresh UCB at 4°C reduced ECFC yield compared with storage at 22°C, while cryopreservation of UCB MNCs significantly reduced ECFC recoveries. To our knowledge, this is the first demonstration that placental weight and temperature of storage prior to processing or culture have significant effects on ECFC frequency in UCB. Our studies further support the evidence that cryopreservation of UCB MNCs compromises ECFC recovery.
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