A targeted neuroglial reporter line generated by homologous recombination in human embryonic stem cells.
A targeted neuroglial reporter line generated by homologous recombination in human embryonic stem cells.
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DOI：
10.1002/stem.129
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发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Xue H;Wu S;Papadeas ST;Spusta S;Swistowska AM;MacArthur CC;Mattson MP;Maragakis NJ;Capecchi MR;Rao MS;Zeng X;Liu Y
In this study we targeted Olig2, a basic helix-loop-helix transcription factor that plays an important role in motoneuron and oligodendrocyte development, in human embryonic stem cell (hESC) line BG01 by homologous recombination. One allele of Olig2 locus was replaced by a GFP cassette with a targeting efficiency of 5.7%. Targeted clone R-Olig2 (like the other clones) retained pluripotency, a typical hESC morphology and a normal parental karyotype 46, XY. Most importantly, GFP expression recapitulated endogenous Olig2 expression when R-Olig2 was induced by sonic hedgehog and retinoic acid, and GFP+ cells could be purified by fluorescence-activated cell sorting (FACS). Consistent with previous reports on rodents, early GFP-expressing cells appeared biased to a neuronal fate whereas late GFP-expressing cells appeared biased to an oligodendrocytic fate. This was corroborated by myoblast coculture, transplantation into the rat spinal cords and whole genome expression profiling. The present work reports an hESC reporter line generated by homologous recombination targeting a neural lineage specific gene, which can be differentiated and sorted to obtain pure neural progenitor populations.
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影响因子：
6.2
作者：
Liu, Y;Rao, MS
通讯作者：
Rao, MS
影响因子：
5.2
作者：
Ren, Caiping;Zhao, Ming;Zhang, Hongbo
通讯作者：
Zhang, Hongbo
DOI：
10.1073/pnas.181340798
发表时间：
2001-09-11
影响因子：
11.1
作者：
Lu, QR;Park, JK;Black, PM
通讯作者：
Black, PM
影响因子：
16.2
作者：
Novitch, BG;Chen, AI;Jessell, TM
通讯作者：
Jessell, TM
影响因子：
16.2
作者：
Ligon, Keith L.;Huillard, Emmanuelle;Rowitch, David H.
通讯作者：
Rowitch, David H.