In vitro and in vivo mapping of drug release after laser ablation thermal therapy with doxorubicin-loaded hollow gold nanoshells using fluorescence and photoacoustic imaging.
In vitro and in vivo mapping of drug release after laser ablation thermal therapy with doxorubicin-loaded hollow gold nanoshells using fluorescence and photoacoustic imaging.
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DOI：
10.1016/j.jconrel.2013.07.020
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发表时间：
2013-11-28
期刊：
影响因子：
--
通讯作者：
Melancon MP
中科院分区：
文献类型：
--
作者：
Lee HJ;Liu Y;Zhao J;Zhou M;Bouchard RR;Mitcham T;Wallace M;Stafford RJ;Li C;Gupta S;Melancon MP
装载阿霉素的中空纳米壳（Dox@PEG-HAuNS）不仅通过介导有效的PTA而且通过化疗来增加光热消融（PTA）的功效，因此具有用于局部抗癌治疗的潜在效用。然而，在激光消融技术过程中实现的Dox释放和温度的体内实时监测之前尚未得到证实。在这项研究中，我们使用荧光光学成像来映射从Dox@PEG-HAuNS和光声成像的Dox的释放，以监测在近红外激光诱导的光热加热在体外和体内实现的肿瘤温度。在体外，用3-W激光处理足以引发Dox从Dox@PEG-HAuNS（1：3：1 wt/wt，1.32×1012个颗粒/mL）中释放。3和6 W的激光功率实现了超过50 °C的烧蚀温度。在接受瘤内或静脉内注射Dox@PEG-HAuNS的4 T1荷瘤裸鼠中，荧光光学成像（发射波长= 600 nm，激发波长= 500 nm）显示，治疗后24小时，表面激光治疗的肿瘤中的荧光强度显著高于未治疗的肿瘤（瘤内p=0.015，静脉内p=0.008）。在静脉内注射Dox@PEG-HAuNS后使用间质激光照射肿瘤获得了类似的结果（在t= 24小时时p=0.002）。光声成像（采集波长= 800 nm）显示，激光治疗导致肿瘤温度大幅升高，从37 °C升高到消融温度超过50 °C。离体分析显示，激光处理的肿瘤的荧光强度是未处理的肿瘤的荧光强度的两倍（p=0.009）。组织学分析证实，与未用激光处理的肿瘤相比，肿瘤内注射Dox@PEG-HAuNS和激光处理引起显著更多的肿瘤坏死（p<0.001）。在这些发现的基础上，我们得出结论，荧光光学成像和光声成像是有前途的方法来评估Dox释放和监测温度，分别在Dox@PEG-HAuNS介导的热消融治疗后。
Doxorubicin-loaded hollow nanoshells (Dox@PEG-HAuNS) increases the efficacy of photothermal ablation (PTA) by not only mediating efficient PTA but also through chemotherapy, and therefore have potential utility for local anticancer therapy. However, in vivo real-time monitoring of Dox release and temperature achieved during the laser ablation technique has not been previously demonstrated before. In this study, we used fluorescence optical imaging to map the release of Dox from Dox@PEG-HAuNS and photoacoustic imaging to monitor the tumor temperature achieved during near-infrared laser–induced photothermal heating in vitro and in vivo. In vitro, treatment with a 3-W laser was sufficient to initiate the release of Dox from Dox@PEG-HAuNS (1:3:1 wt/wt, 1.32×1012 particles/mL). Laser powers of 3 and 6 W achieved ablative temperatures of more than 50 °C. In 4T1 tumor–bearing nude mice that received intratumoral or intravenous injections of Dox@PEG-HAuNS, fluorescence optical imaging (emission wavelength = 600 nm, excitation wavelength = 500 nm) revealed that the fluorescence intensity in surface laser–treated tumors 24 h after treatment was significantly higher than that in untreated tumors (p=0.015 for intratumoral, p=0.008 for intravenous). Similar results were obtained using an interstitial laser to irradiate tumors following the intravenous injection of Dox@PEG-HAuNS (p=0.002 at t=24h). Photoacoustic imaging (acquisition wavelength = 800 nm) revealed that laser treatment caused a substantial increase in tumor temperature, from 37 °C to ablative temperatures of more than 50 °C. Ex vivo analysis revealed that the fluorescence intensity of laser-treated tumors was twice as high as that of untreated tumors (p=0.009). Histological analysis confirmed that intratumoral injection of Dox@PEG-HAuNS and laser treatment caused significantly more tumor necrosis compared to tumors that were not treated with laser (p<0.001). On the basis of these findings, we conclude that fluorescence optical imaging and photoacoustic imaging are promising approaches to assessing Dox release and monitoring temperature, respectively, after Dox@PEG-HAuNS–mediated thermal ablation therapy.
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空心硫化铜纳米颗粒介导的透皮药物递送。
影响因子：
13.3
作者：
Ramadan, Samy;Guo, Liangran;Li, Yajuan;Yan, Bingfang;Lu, Wei
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影响因子：
3.5
作者：
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影响因子：
3.4
作者：
Cook JR;Bouchard RR;Emelianov SY
通讯作者：
Emelianov SY
影响因子：
2.8
作者：
Chen, Yun-Sheng;Frey, Wolfgang;Emelianov, Stanislav
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DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL