Synergistic effects of silver ions and metformin against enterococcus faecalis under high-glucose conditions in vitro.
Synergistic effects of silver ions and metformin against enterococcus faecalis under high-glucose conditions in vitro.
复制标题
高糖条件下银离子与二甲双胍对粪肠球菌的协同作用
DOI：
10.1186/s12866-021-02291-2
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发表时间：
2021-09-29
期刊：
影响因子：
4.2
通讯作者：
Fan B
中科院分区：
文献类型：
--
作者：
Wu X;Fan W;Fan B
背景
研究银离子（Ag+）与盐酸二甲双胍（Met）对粪肠球菌（E.粪便）在正常或高糖条件下。
结果
采用最低抑菌浓度、最低杀菌浓度、生长曲线和菌落形成单位等方法评价Ag+和Met对大肠杆菌的抑菌效果。在脑心浸液肉汤中添加或不添加额外的葡萄糖。Ag+和Met对4周E.还测试了人牙本质切片上的粪便生物膜。使用CCK-8测定法对MC 3 T3-E1成骨细胞进行细胞毒性试验。结果表明，E.在高糖条件下，粪肠球菌对药物治疗表现出更高的抵抗力。Ag+（40 μg/mL）和Met（3.2%或6.4%）对大肠杆菌和大肠杆菌均有较强的抗菌活性。牙本质切片上的粪便和生物膜，具有低细胞毒性。
结论
Met可增强Ag+对大肠杆菌和生物膜E的杀菌作用。在正常或高葡萄糖条件下对粪肠球菌具有低细胞毒性。需要进行进一步的分子研究以了解Met和Ag+之间协同活性的机制。
Background
This study aimed to evaluate the synergistic antibacterial activities of silver ions (Ag+) and metformin hydrochloride (Met) against Enterococcus faecalis (E. faecalis) under normal or high-glucose conditions.
Results
The minimum inhibitory concentration, minimum bactericidal concentration, growth curves, and colony-forming units were used to evaluate the antibacterial effects of Ag+ and Met on planktonic E. faecalis in Brain Heart Infusion broth with or without additional glucose. The influences of Ag+ and Met on four weeks E. faecalis biofilm on human dentin slices was also tested. Cytotoxicity was tested on MC3T3-E1 osteoblastic cells using CCK-8 assays. The results indicated that E. faecalis showed higher resistance to drug treatment under high-glucose conditions. Ag+ (40 μg/mL) plus Met (3.2% or 6.4%) showed enhanced antibacterial activities against both planktonic E. faecalis and biofilm on dentin slices, with low cytotoxicity.
Conclusions
Met enhanced the bactericidal effects of Ag+ against both planktonic and biofilm E. faecalis under normal or high-glucose conditions with low cytotoxicity. Further molecular studies are needed to be conducted to understand the mechanisms underlying the synergistic activity between Met and Ag+.
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影响因子：
4.2
作者：
McHugh, CP;Zhang, P;Eleazer, PD
通讯作者：
Eleazer, PD
影响因子：
0.7
作者：
Katva S;Das S;Moti HS;Jyoti A;Kaushik S
通讯作者：
Kaushik S
DOI：
10.1016/s1079-2104(03)00166-5
发表时间：
2003-11-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Basrani, B;Tjäderhane, L;Friedman, S
通讯作者：
Friedman, S
影响因子：
3.2
作者：
Li, XZ;Nikaido, H;Williams, KE
通讯作者：
Williams, KE
影响因子：
20.1
作者：
Cameron AR;Morrison VL;Levin D;Mohan M;Forteath C;Beall C;McNeilly AD;Balfour DJ;Savinko T;Wong AK;Viollet B;Sakamoto K;Fagerholm SC;Foretz M;Lang CC;Rena G
通讯作者：
Rena G