Scaffold-based developmental tissue engineering strategies for ectodermal organ regeneration.
Scaffold-based developmental tissue engineering strategies for ectodermal organ regeneration.
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外胚层器官再生的支架型发育组织工程策略。
DOI：
10.1016/j.mtbio.2021.100107
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Celiz AD
中科院分区：
文献类型：
--
作者：
Contessi Negrini N;Angelova Volponi A;Higgins CA;Sharpe PT;Celiz AD
组织工程（TE）是一门多学科的研究领域，旨在再生、修复或替换受损组织和器官。经典的TE方法结合支架、细胞和可溶性因子来制造模拟原生组织的结构。然而，迄今为止，由于所获得的构造体缺乏令人满意的生物形态学和生物功能特征，经典TE方法在临床翻译中取得的成功有限。发育性TE已经成为一种新的TE模式，通过模仿胚胎中导致组织/器官生成的形态发生过程来获得具有正确生物形态和生物功能的组织和器官。例如，外胚层附属物在体内通过上皮和间质之间的连续相互作用形成，这一过程被称为次生诱导。对这些复杂的相互作用进行精细的人工复制，可能会导致要再生的组织/器官的制造。据报道，TE在体外和体内成功应用于外胚层附属物，如牙齿、毛囊和腺体。发育性TE策略需要准确选择细胞来源、支架和细胞培养配置，以允许体内形态发生线索的正确复制。在此，我们通过回顾迄今为止在牙齿、毛囊、唾液腺和泪腺的发育性TE 3D支架方面取得的成就，描述和讨论了这种TE范式的出现，特别关注生物材料的选择和细胞培养结构。发育组织工程（TE）模拟器官的形态发生来再生器官。牙齿，毛囊和腺体成功再生发育TE。3D细胞种子/3D细胞负载支架是发育性TE器官再生的基础。细胞来源、共培养和支架设计决定了发育性TE的成功。
Tissue engineering (TE) is a multidisciplinary research field aiming at the regeneration, restoration, or replacement of damaged tissues and organs. Classical TE approaches combine scaffolds, cells and soluble factors to fabricate constructs mimicking the native tissue to be regenerated. However, to date, limited success in clinical translations has been achieved by classical TE approaches, because of the lack of satisfactory biomorphological and biofunctional features of the obtained constructs. Developmental TE has emerged as a novel TE paradigm to obtain tissues and organs with correct biomorphology and biofunctionality by mimicking the morphogenetic processes leading to the tissue/organ generation in the embryo. Ectodermal appendages, for instance, develop in vivo by sequential interactions between epithelium and mesenchyme, in a process known as secondary induction. A fine artificial replication of these complex interactions can potentially lead to the fabrication of the tissues/organs to be regenerated. Successful developmental TE applications have been reported, in vitro and in vivo, for ectodermal appendages such as teeth, hair follicles and glands. Developmental TE strategies require an accurate selection of cell sources, scaffolds and cell culture configurations to allow for the correct replication of the in vivo morphogenetic cues. Herein, we describe and discuss the emergence of this TE paradigm by reviewing the achievements obtained so far in developmental TE 3D scaffolds for teeth, hair follicles, and salivary and lacrimal glands, with particular focus on the selection of biomaterials and cell culture configurations. Developmental tissue engineering (TE) mimics morphogenesis to regenerate organs. Tooth, hair follicle, and glands successfully regenerated by developmental TE. 3D cell-seeded/3D cell-laden scaffolds fundamental to regenerate organs by developmental TE. Cell source, coculture and scaffold design determine success of developmental TE.
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