A systematic comparison between collagen-induced arthritis and pristane-induced arthritis in Dark Agouti rats.
A systematic comparison between collagen-induced arthritis and pristane-induced arthritis in Dark Agouti rats.
复制标题
在暗刺豚鼠中胶原诱导的关节炎和降植烷诱导的关节炎之间的系统比较。
DOI：
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发表时间：
2010-07
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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作者：
目标
胶原诱导的关节炎（CIA）和降植烷诱导的关节炎（PIA）都是常用的类风湿关节炎（RA）大鼠模型。本研究的目的是系统地比较暗刺豚鼠 (DA) 大鼠中 CIA 和 PIA 的差异。
方法
通过皮内注射C型胶原蛋白免疫DA大鼠来诱导CIA，通过皮下注射降植烷来诱导PIA。对关节炎进行宏观和微观评估。采用格里斯反应法测定血浆中一氧化氮（NO）水平。采用酶联免疫吸附法检测血浆自身免疫抗体，包括C特异性IgG抗体（抗C IgG）、环瓜氨酸肽特异性IgG抗体（抗CCP IgG）、IgM和IgG类风湿因子（IgM RF和IgG RF）。
结果
PIA大鼠的发病早于CIA大鼠。 PIA大鼠的受累部位主要是腕/踝关节和掌指/跖趾(MTP)关节，而CIA大鼠的受累部位主要是远端指间(DIP)关节。 PIA大鼠血浆NO水平升高，而CIA大鼠血浆抗C IgG、抗CCP IgG、IgM RF和IgG RF水平升高。 CIA大鼠的肾透明管型比对照组大鼠更常见，PIA组为9/12，CIA组为8/8，对照组为4/8。
结论
PIA主要累及腕/踝关节和MCP/MTP关节，炎症较严重，几乎不累及其他器官；而CIA则主要影响DIP关节，血浆中自身免疫抗体较多，肾脏中常出现透明管型。这些发现将有助于选择 RA 动物模型。
OBJECTIVES
Both collagen-induced arthritis (CIA) and pristane-induced arthritis (PIA) are commonly used rat models of rheumatoid arthritis (RA). The aim of this study was systematically to compare the differences between CIA and PIA in Dark Agouti (DA) rats.
METHODS
The CIA was induced by immunising DA rats intradermally with collagen type (C) and PIA was induced by injecting subcutaneously with pristane. The arthritis was evaluated macroscopically and microscopically. Nitric oxide (NO) level of plasma was determined by Griess reaction method. Plasma autoimmune antibodies, including C specific IgG antibody (anti-C IgG), cyclic citrullinated peptide specific IgG antibody (anti-CCP IgG), IgM and IgG rheumatoid factors (IgM RF and IgG RF), were detected by the enzyme-linked immunosorbent assay.
RESULTS
The onset of PIA rats was earlier than that of CIA rats. The involved sites of PIA rats were mostly wrist/ankle and metacarpophalangeal/metatarsophalangeal (MCP/MTP) joints while those of CIA rats were primarily distal interphalangeal (DIP) joints. NO level of plasma was increased in PIA rats, as anti-C IgG, anti-CCP IgG, IgM RF and IgG RF levels of plasma were increased in CIA rats. The kidney hyaline casts were more frequent in CIA rats than in control rats, with 9/12 in PIA group, 8/8 in CIA, and 4/8 in control, respectively.
CONCLUSIONS
PIA mainly affected wrist/ankle joints and MCP/MTP joints, had more severe inflammation and hardly involved other organs; while CIA mostly influenced DIP joints, had more autoimmune antibodies in plasma, and always showed hyaline casts in kidney. These findings will be useful to select the animal model of RA.
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影响因子：
4
作者：
Peng Zhang;Dong Han;Ting‐ting Tang;Xiaoling Zhang;K. Dai
通讯作者：
Peng Zhang;Dong Han;Ting‐ting Tang;Xiaoling Zhang;K. Dai
影响因子：
6
作者：
Nacionales, DC;Kelly, KM;Reeves, WH
通讯作者：
Reeves, WH
影响因子：
15.3
作者：
STUART, JM;CREMER, MA;KANG, AH
通讯作者：
KANG, AH
影响因子：
3.7
作者：
C. Jeon;J. Ahn;Chai Jy;Kim Hj;Bae Ek;S. Park;Chow Ey;H. Cha;Ahn Ks;Koh Em
通讯作者：
C. Jeon;J. Ahn;Chai Jy;Kim Hj;Bae Ek;S. Park;Chow Ey;H. Cha;Ahn Ks;Koh Em
影响因子：
4.6
作者：
Harris, HE;Liljestrom, M;Klareskog, L
通讯作者：
Klareskog, L