A spatial temporal analysis of the Fusarium graminearum transcriptome during symptomless and symptomatic wheat infection.
A spatial temporal analysis of the Fusarium graminearum transcriptome during symptomless and symptomatic wheat infection.
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DOI：
10.1111/mpp.12564
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发表时间：
2017-12
影响因子：
4.9
通讯作者：
Hammond-Kosack KE
中科院分区：
文献类型：
--
作者：
Brown NA;Evans J;Mead A;Hammond-Kosack KE
小麦赤霉病是世界范围内危害最严重的作物病害之一。在这里，禾谷镰刀菌的转录组学研究揭示了一种新的无症状小麦感染模型。预测的代谢状态和分泌组的禾谷镰刀菌是不同的无症状和症状的小麦组织。转录的基因参与生物合成的真菌毒素，脱氧雪腐镰刀菌烯醇，加上其他特征和推定的次级代谢物集群增加丰富的无菌组织。转录本编码的基因的不同群体的推定分泌效应增加内无瘤或有症状的组织。许多致病性相关基因转录本和代表影响毒力的PHI碱基突变的转录本在无菌组织中增加。相比之下，水解碳水化合物活性酶（CAZyme）和脂肪酶基因转录物表现出不同的表达模式，导致疾病症状发展期间转录物丰度升高。与现有数据集的全基因组比较证实，在小麦花组织内，在单个时间点，不同的感染阶段共存，它们在空间上是分开的，让人想起早期和晚期感染。这项研究提供了新的见解结合时空协调的功能特点和假设的毒力策略。
Fusarium head blight of wheat is one of the most serious and hazardous crop diseases worldwide. Here, a transcriptomic investigation of Fusarium graminearum reveals a new model for symptomless and symptomatic wheat infection. The predicted metabolic state and secretome of F. graminearum were distinct within symptomless and symptomatic wheat tissues. Transcripts for genes involved in the biosynthesis of the mycotoxin, deoxynivalenol, plus other characterized and putative secondary metabolite clusters increased in abundance in symptomless tissue. Transcripts encoding for genes of distinct groups of putative secreted effectors increased within either symptomless or symptomatic tissue. Numerous pathogenicity‐associated gene transcripts and transcripts representing PHI‐base mutations that impacted on virulence increased in symptomless tissue. In contrast, hydrolytic carbohydrate‐active enzyme (CAZyme) and lipase gene transcripts exhibited a different pattern of expression, resulting in elevated transcript abundance during the development of disease symptoms. Genome‐wide comparisons with existing datasets confirmed that, within the wheat floral tissue, at a single time point, different phases of infection co‐exist, which are spatially separated and reminiscent of both early and late infection. This study provides novel insights into the combined spatial temporal coordination of functionally characterized and hypothesized virulence strategies.
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对坏死性植物病原体的菌核病菌核和辣椒粉预测的分析的种间比较分析。
影响因子：
3.7
作者：
Heard S;Brown NA;Hammond-Kosack K
通讯作者：
Hammond-Kosack K
影响因子：
4.9
作者：
Desmond, Olivia J.;Manners, John M.;Kazan, Kemal
通讯作者：
Kazan, Kemal
影响因子：
9.4
作者：
Cuzick, Alayne;Urban, Martin;Hammond-Kosack, Kim
通讯作者：
Hammond-Kosack, Kim
影响因子：
5.1
作者：
Bahadoor, Adilah;Schneiderman, Danielle;Harris, Linda J.
通讯作者：
Harris, Linda J.
影响因子：
3
作者：
Brown, Neil Andrew;Ries, Laure Nicolas Annick;Goldman, Gustavo Henrique
通讯作者：
Goldman, Gustavo Henrique