Terminals of the major thalamic input to visual cortex are devoid of synapsin proteins.
Terminals of the major thalamic input to visual cortex are devoid of synapsin proteins.
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DOI：
10.1016/j.neuroscience.2013.03.031
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发表时间：
2013-07-23
期刊：
影响因子：
3.3
通讯作者：
Heggelund, P.
中科院分区：
文献类型：
--
作者：
Owe, S. G.;Erisir, A.;Heggelund, P.
突触蛋白是神经末梢蛋白，与突触传递和多种形式的突触可塑性的关键因素有关。虽然突触蛋白通常被认为在突触末端中普遍存在，但它们是否被排除在某些类型的末端之外是令人感兴趣的。在视觉通路中，光感受器和双极细胞末梢以及视网膜突触中缺乏突触蛋白。这些是视觉通路中前三个前馈突触的末端，这意味着缺乏突触蛋白可能是为突触后神经元提供主要驱动活动的末端的共同属性。为了进一步研究这个想法，我们研究了第四个驱动突触，即视皮层中的丘脑皮层突触，使用谷氨酸末端抗体标记物抗VGluT1和VGluT2、抗突触蛋白I和II，以及共聚焦显微镜来分析这些蛋白质在末端的共定位。我们还使用预嵌入免疫细胞化学标记，然后通过电子显微镜来研究含有突触蛋白或 VGluT2 的末端之间的形态相似性或差异。在视觉皮层中，突触蛋白与非 TC 神经元标记 VGluT1 广泛一致，而丘脑皮质末端标记 VGluT2 和突触蛋白重叠很少。从形态上看，突触蛋白染色的末端比未染色的末端小，而 VGluT2 阳性丘脑皮质末端构成了皮质中最大的末端。突触蛋白和 VGluT2 阳性末端之间的大小差异以及互补的染色模式表明丘脑皮质突触缺乏突触蛋白，并支持传入感觉信息在没有突触蛋白参与的情况下一致传递的假设。此外，VGluT2 和突触蛋白共定位于其他大脑结构中，这表明缺乏突触蛋白并不是包含 VGluT2 终端的特性，而是视觉系统中主要驱动终端的特性。
Synapsins are nerve-terminal proteins that are linked to synaptic transmission and key factors in several forms of synaptic plasticity. While synapsins are generally assumed to be ubiquitous in synaptic terminals, whether they are excluded from certain types of terminals is of interest. In the visual pathway, synapsins are lacking in photoreceptor and bipolar cell terminals as well as in retinogeniculate synapses. These are the terminals of the first three feedforward synapses in the visual pathway, implying that lack of synapsins may be a common property of terminals that provide the primary driver activity onto their postsynaptic neurons. To further investigate this idea, we studied the fourth driver synapse, thalamocortical synapses in visual cortex, using glutamatergic terminal antibody markers anti-VGluT1 and VGluT2, anti-Synapsin I and II, and confocal microscopy to analyze co-localization of these proteins in terminals. We also used pre-embedding immunocytochemical labeling followed by electron microscopy to investigate morphological similarities or differences between terminals containing synapsins or VGluT2. In visual cortex, synapsin coincided extensively with non-TC-neuron marker, VGluT1, while thalamocortical terminal marker VGluT2 and synapsin overlap was sparse. Morphologically, synapsin-stained terminals were smaller than non-stained, while VGluT2 positive thalamocortical terminals constituted the largest terminals in cortex. The size discrepancy between synapsin- and VGluT2-positive terminals, together with the complementary staining patterns, indicate that thalamocortical synapses are devoid of synapsins, and support the hypothesis that afferent sensory information is consistently transmitted without involvement of synapsins. Furthermore, VGluT2 and synapsins were colocalized in other brain structures, suggesting that lack of synapsins is not a property of VGluT2 containing terminals, but a property of primary driver terminals in the visual system.
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丘脑皮质突触的快速结构重塑使小鼠初级视觉皮层的经验依赖性功能可塑性。
DOI：
10.1523/jneurosci.1248-10.2010
发表时间：
2010-07-21
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Coleman JE;Nahmani M;Gavornik JP;Haslinger R;Heynen AJ;Erisir A;Bear MF
通讯作者：
Bear MF
影响因子：
56.9
作者：
Fremeau, RT;Kam, K;Edwards, RH
通讯作者：
Edwards, RH
DOI：
10.1073/pnas.95.8.4667
发表时间：
1998-04-14
影响因子：
11.1
作者：
Kao, HT;Porton, B;Greengard, P
通讯作者：
Greengard, P
影响因子：
16.2
作者：
Fremeau, RT;Troyer, MD;Edwards, RH
通讯作者：
Edwards, RH
影响因子：
5.3
作者：
da Costa, Nuno Macarico;Fuersinger, David;Martin, Kevan A. C.
通讯作者：
Martin, Kevan A. C.